Sökning: WFRF:(Wu Yitong) >
PP2A-B' holoenzyme ...
PP2A-B' holoenzyme substrate recognition, regulation and role in cytokinesis
-
- Wu, Cheng-Guo (författare)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA.; Univ Wisconsin Madison, Biophys Program, Madison, WI 53706 USA.; DuPont Ind Biosci, Genencor Div, 925 Page Mill Rd, Palo Alto, CA 94304 USA
-
- Chen, Hui (författare)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA.; DuPont Ind Biosci, Genencor Div, 925 Page Mill Rd, Palo Alto, CA 94304 USA
-
- Guo, Feng (författare)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA.; DuPont Ind Biosci, Genencor Div, 925 Page Mill Rd, Palo Alto, CA 94304 USA.; Univ Pittsburgh, Dept Microbiol & Mol Genet, Pittsburgh, PA USA
-
visa fler...
-
- Yadav, Vikash K (författare)
- Uppsala universitet,Institutionen för kemi - BMC,DuPont Ind Biosci, Genencor Div, 925 Page Mill Rd, Palo Alto, CA 94304 USA,Ivarsson
-
- Mcilwain, Sean J (författare)
- Univ Wisconsin Madison, Sch Med & Publ Hlth, Biostat & Med Informat, Wisconsin Inst Med Res, Madison, WI USA
-
- Rowse, Michael (författare)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA
-
- Choudhary, Alka (författare)
- Univ Wisconsin Madison, Sch Med & Publ Hlth, Dept Med, UW Carbone Canc Ctr,Hematol Oncol, Madison, WI USA
-
- Lin, Ziqing (författare)
- Sch Med & Publ Hlth, Dept Cell & Regenerat Biol, Human Prote Program, Madison, WI USA
-
- Li, Yitong (författare)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA
-
- Gu, Tingjia (författare)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA
-
- Zheng, Aiping (författare)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA.; Univ Pittsburgh, Dept Microbiol & Mol Genet, Pittsburgh, PA USA
-
- Xu, Qingge (författare)
- Sch Med & Publ Hlth, Dept Cell & Regenerat Biol, Human Prote Program, Madison, WI USA
-
- Lee, Woojong (författare)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA
-
- Resch, Eduard (författare)
- Fraunhofer Inst Mol Biol & Appl Ecol IME, Project Grp Translat Med & Pharmacol TMP, Frankfurt, Germany
-
- Johnson, Benjamin (författare)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA
-
- Day, Jenny (författare)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA
-
- Ge, Ying (författare)
- Sch Med & Publ Hlth, Dept Cell & Regenerat Biol, Human Prote Program, Madison, WI USA
-
- Ong, Irene M (författare)
- Univ Wisconsin Madison, Sch Med & Publ Hlth, Biostat & Med Informat, Wisconsin Inst Med Res, Madison, WI USA
-
- Burkard, Mark E (författare)
- Univ Wisconsin Madison, Sch Med & Publ Hlth, Dept Med, UW Carbone Canc Ctr,Hematol Oncol, Madison, WI USA
-
- Ivarsson, Ylva (författare)
- Uppsala universitet,Biokemi,Ivarsson
-
- Xing, Yongna (författare)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA.; Univ Wisconsin Madison, Biophys Program, Madison, WI 53706 USA
-
visa färre...
-
Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA; Univ Wisconsin Madison, Biophys Program, Madison, WI 53706 USA.; DuPont Ind Biosci, Genencor Div, 925 Page Mill Rd, Palo Alto, CA 94304 USA Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA.; DuPont Ind Biosci, Genencor Div, 925 Page Mill Rd, Palo Alto, CA 94304 USA (creator_code:org_t)
- 2017-08-08
- 2017
- Engelska.
-
Ingår i: Cell Discovery. - : Springer Science and Business Media LLC. - 2056-5968. ; 3
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://uu.diva-port... (primary) (Raw object)
-
https://www.nature.c...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Protein phosphatase 2A (PP2A) is a major Ser/Thr phosphatase; it forms diverse heterotrimeric holoenzymes that counteract kinase actions. Using a peptidome that tiles the disordered regions of the human proteome, we identified proteins containing [LMFI]xx[ILV]xEx motifs that serve as interaction sites for B'-family PP2A regulatory subunits and holoenzymes. The B'-binding motifs have important roles in substrate recognition and in competitive inhibition of substrate binding. With more than 100 novel ligands identified, we confirmed that the recently identified LxxIxEx B'α-binding motifs serve as common binding sites for B' subunits with minor variations, and that S/T phosphorylation or D/E residues at positions 2, 7, 8 and 9 of the motifs reinforce interactions. Hundreds of proteins in the human proteome harbor intrinsic or phosphorylation-responsive B'-interaction motifs, and localize at distinct cellular organelles, such as midbody, predicting kinase-facilitated recruitment of PP2A-B' holoenzymes for tight spatiotemporal control of phosphorylation at mitosis and cytokinesis. Moroever, Polo-like kinase 1-mediated phosphorylation of Cyk4/RACGAP1, a centralspindlin component at the midbody, facilitates binding of both RhoA guanine nucleotide exchange factor (epithelial cell transforming sequence 2 (Ect2)) and PP2A-B' that in turn dephosphorylates Cyk4 and disrupts Ect2 binding. This feedback signaling loop precisely controls RhoA activation and specifies a restricted region for cleavage furrow ingression. Our results provide a framework for further investigation of diverse signaling circuits formed by PP2A-B' holoenzymes in various cellular processes.
Ämnesord
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Nyckelord
- CIP2A
- PP2A-B′ holoenzyme
- SLiMs
- centrosome
- cytokinesis
- midbody
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Wu, Cheng-Guo
-
Chen, Hui
-
Guo, Feng
-
Yadav, Vikash K
-
Mcilwain, Sean J
-
Rowse, Michael
-
visa fler...
-
Choudhary, Alka
-
Lin, Ziqing
-
Li, Yitong
-
Gu, Tingjia
-
Zheng, Aiping
-
Xu, Qingge
-
Lee, Woojong
-
Resch, Eduard
-
Johnson, Benjami ...
-
Day, Jenny
-
Ge, Ying
-
Ong, Irene M
-
Burkard, Mark E
-
Ivarsson, Ylva
-
Xing, Yongna
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Bioinformatik oc ...
- Artiklar i publikationen
-
Cell Discovery
- Av lärosätet
-
Uppsala universitet