Emerging microbiota during cold storage and temperature abuse of ready-to-eat salad​

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Söderqvist, KarinSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health,Swedish University of Agricultural Sciences, Uppsala, Sweden (författare)

Emerging microbiota during cold storage and temperature abuse of ready-to-eat salad

Artikel/kapitelEngelska2017

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2017-06-06
Informa UK Limited,2017
electronicrdacarrier

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LIBRIS-ID:oai:DiVA.org:uu-339036
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-339036URI
https://doi.org/10.1080/20008686.2017.1328963DOI
https://res.slu.se/id/publ/84169URI

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Språk:engelska
Sammanfattning på:engelska

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Introduction: Ready-to-eat (RTE) leafy vegetables have a natural leaf microbiota that changes during different processing and handling steps from farm to fork. The objectives of this study were (i) to compare the microbiota of RTE baby spinach and mixed-ingredient salad before and after seven days of storage at 8°C or 15°C; (ii) to explore associations between bacterial communities and the foodborne pathogens Listeria monocytogenes, pathogenic Yersinia enterocolitica, and pathogen model organism Escherichia coli O157:H7 gfp+ when experimentally inoculated into the salads before storage; and (iii) to investigate if bacterial pathogens may be detected in the 16S rRNA amplicon dataset. Material and methods: The microbiota was studied by means of Illumina 16S rRNA amplicon sequencing. Subsets of samples were inoculated with low numbers (50-100 CFU g(-1)) of E. coli O157:H7 gfp+, pathogenic Y. enterocolitica or L. monocytogenes before storage. Results and discussion: The composition of bacterial communities changed during storage of RTE baby spinach and mixed-ingredient salad, with Pseudomonadales as the most abundant order across all samples. Although pathogens were present at high viable counts in some samples, they were only detected in the community-wide dataset in samples where they represented approximately 10% of total viable counts. Positive correlations were identified between viable counts of inoculated strains and the abundance of Lactobacillales, Enterobacteriales, and Bacillales, pointing to positive interactions or similar environmental driver variables that may make it feasible to use such bacterial lineages as indicators of microbial health hazards in leafy vegetables. The data from this study contribute to a better understanding of the bacteria present in RTE salads and may help when developing new types of biocontrol agents.

Ämnesord och genrebeteckningar

NATURVETENSKAP Biologi hsv//swe
NATURAL SCIENCES Biological Sciences hsv//eng
LANTBRUKSVETENSKAPER Veterinärmedicin Patobiologi hsv//swe
AGRICULTURAL SCIENCES Veterinary Science Pathobiology hsv//eng
Baby spinach
Escherichia coli O157:H7
Listeria monocytogenes
mixed-ingredient salad
pathogenic Yersinia enterocolitica
phyllosphere

Biuppslag (personer, institutioner, konferenser, titlar ...)

Ahmed Osman, OmneyaUppsala universitet,Limnologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)omnah486 (författare)
Wolff, CeciliaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health,Swedish University of Agricultural Sciences, Uppsala, Sweden(Swepub:slu)51071 (författare)
Bertilsson, StefanUppsala universitet,Limnologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)stber839 (författare)
Vågsholm, IvarSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health,Swedish University of Agricultural Sciences, Uppsala, Sweden(Swepub:slu)48570 (författare)
Boqvist, SofiaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health,Swedish University of Agricultural Sciences, Uppsala, Sweden(Swepub:slu)47597 (författare)
Sveriges lantbruksuniversitetInst för biomedicin och veterinär folkhälsovetenskap (creator_code:org_t)
Sveriges lantbruksuniversitet

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Ingår i:Infection Ecology & Epidemiology: Informa UK Limited7:12000-8686

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Av författaren/redakt...: Söderqvist, Kari ...; Ahmed Osman, Omn ...; Wolff, Cecilia; Bertilsson, Stef ...; Vågsholm, Ivar; Boqvist, Sofia

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NATURVETENSKAP: NATURVETENSKAP; och Biologi

LANTBRUKSVETENSKAPER: LANTBRUKSVETENSK ...; och Veterinärmedicin; och Patobiologi

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