SwePub
Sök i LIBRIS databas

  Extended search

WFRF:(Mainez Jessica)
 

Search: WFRF:(Mainez Jessica) > (2009) > Role of CXCR4/SDF-1...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Bertran, Esther(IDIBELL), Barcelona, Spain (author)

Role of CXCR4/SDF-1 alpha in the migratory phenotype of hepatoma cells that have undergone epithelial-mesenchymal transition in response to the transforming growth factor-beta.

  • Article/chapterEnglish2009

Publisher, publication year, extent ...

  • Elsevier BV,2009
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-339279
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-339279URI
  • https://doi.org/10.1016/j.cellsig.2009.06.006DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Treatment of FaO rat hepatoma cells with TGF-beta selects cells that survive to its apoptotic effect and undergo epithelial-mesenchymal transitions (EMT). We have established a cell line (T beta T-FaO, from TGF-beta-treated FaO) that shows a mesenchymal, de-differentiated, phenotype in the presence of TGF-beta and is refractory to its suppressor effects. In the absence of this cytokine, cells revert to an epithelial phenotype in 3-4 weeks and recover the response to TGF-beta. T beta T-FaO show higher capacity to migrate than that observed in the parental FaO cells. We found that FaO cells express low levels of CXCR4 and do not respond to SDF-1 alpha. However, TGF-beta up-regulates CXCR4, through a NF kappaB-dependent mechanism, and T beta T-FaO cells show elevated levels of CXCR4, which is located in the presumptive migration front. A specific CXCR4 antagonist (AMD3100) attenuates the migratory capacity of T beta T-FaO cells on collagen gels. Extracellular SDF-1 alpha activates the ERKs pathway in T beta T-FaO, but not in FaO cells, increasing cell scattering and protecting cells from apoptosis induced by serum deprivation. Targeted knock-down of CXCR4 with specific siRNA blocks the T beta T-FaO response to SDF-1 alpha. Thus, the SDF-1/CXCR4 axis might play an important role in mediating cell migration and survival after a TGF-beta-induced EMT in hepatoma cells.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Caja, Laia(IDIBELL), Barcelona, Spain(Swepub:uu)laica659 (author)
  • Navarro, Estanis(IDIBELL), Barcelona, Spain (author)
  • Sancho, Patricia(IDIBELL), Barcelona, Spain (author)
  • Mainez, Jèssica(IDIBELL), Barcelona, Spain (author)
  • Murillo, Miguel M(IDIBELL), Barcelona, Spain (author)
  • Vinyals, Antonia(IDIBELL), Barcelona, Spain (author)
  • Fabra, Angels(IDIBELL), Barcelona, Spain (author)
  • Fabregat, Isabel(IDIBELL), Barcelona, Spain (author)
  • (IDIBELL), Barcelona, Spain (creator_code:org_t)

Related titles

  • In:Cellular Signalling: Elsevier BV21:11, s. 1595-16060898-65681873-3913

Internet link

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Search outside SwePub

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view