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Role of CXCR4/SDF-1...
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Bertran, Esther(IDIBELL), Barcelona, Spain
(author)
Role of CXCR4/SDF-1 alpha in the migratory phenotype of hepatoma cells that have undergone epithelial-mesenchymal transition in response to the transforming growth factor-beta.
- Article/chapterEnglish2009
Publisher, publication year, extent ...
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Elsevier BV,2009
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-339279
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-339279URI
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https://doi.org/10.1016/j.cellsig.2009.06.006DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Treatment of FaO rat hepatoma cells with TGF-beta selects cells that survive to its apoptotic effect and undergo epithelial-mesenchymal transitions (EMT). We have established a cell line (T beta T-FaO, from TGF-beta-treated FaO) that shows a mesenchymal, de-differentiated, phenotype in the presence of TGF-beta and is refractory to its suppressor effects. In the absence of this cytokine, cells revert to an epithelial phenotype in 3-4 weeks and recover the response to TGF-beta. T beta T-FaO show higher capacity to migrate than that observed in the parental FaO cells. We found that FaO cells express low levels of CXCR4 and do not respond to SDF-1 alpha. However, TGF-beta up-regulates CXCR4, through a NF kappaB-dependent mechanism, and T beta T-FaO cells show elevated levels of CXCR4, which is located in the presumptive migration front. A specific CXCR4 antagonist (AMD3100) attenuates the migratory capacity of T beta T-FaO cells on collagen gels. Extracellular SDF-1 alpha activates the ERKs pathway in T beta T-FaO, but not in FaO cells, increasing cell scattering and protecting cells from apoptosis induced by serum deprivation. Targeted knock-down of CXCR4 with specific siRNA blocks the T beta T-FaO response to SDF-1 alpha. Thus, the SDF-1/CXCR4 axis might play an important role in mediating cell migration and survival after a TGF-beta-induced EMT in hepatoma cells.
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Caja, Laia(IDIBELL), Barcelona, Spain(Swepub:uu)laica659
(author)
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Navarro, Estanis(IDIBELL), Barcelona, Spain
(author)
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Sancho, Patricia(IDIBELL), Barcelona, Spain
(author)
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Mainez, Jèssica(IDIBELL), Barcelona, Spain
(author)
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Murillo, Miguel M(IDIBELL), Barcelona, Spain
(author)
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Vinyals, Antonia(IDIBELL), Barcelona, Spain
(author)
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Fabra, Angels(IDIBELL), Barcelona, Spain
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Fabregat, Isabel(IDIBELL), Barcelona, Spain
(author)
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(IDIBELL), Barcelona, Spain
(creator_code:org_t)
Related titles
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In:Cellular Signalling: Elsevier BV21:11, s. 1595-16060898-65681873-3913
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