WFRF:(Yefanov Oleksandr)
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Mix-and-diffuse serial synchrotron crystallography
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- Beyerlein, Kenneth R. (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Elect Laser Sci, Notkestr 85, D-22607 Hamburg, Germany.
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- Dierksmeyer, Dennis (author)
- Deutsch Elektronen Synchrotron DESY, Photon Sci, Hamburg, Germany.
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- Mariani, Valerio (author)
- Deutsch Elektronen Synchrotron DESY, Ctr Free Elect Laser Sci, Notkestr 85, D-22607 Hamburg, Germany.
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- Deutsch Elektronen Synchrotron DESY, Ctr Free Elect Laser Sci, Notkestr 85, D-22607 Hamburg, Germany Deutsch Elektronen Synchrotron DESY, Photon Sci, Hamburg, Germany. (creator_code:org_t)
- INT UNION CRYSTALLOGRAPHY, 2017
- 2017
- English.
- In: IUCrJ. - : INT UNION CRYSTALLOGRAPHY. - 2052-2525. ; 4:6, s. 769-777
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron lasers and subsequently at synchrotron light sources allows new approaches to tackle this challenge. Here, a new polyimide tape drive designed for mix-and-diffuse serial crystallography experiments is reported. The structure of lysozyme bound by the competitive inhibitor chitotriose was determined using this device in combination with microfluidic mixers. The electron densities obtained from mixing times of 2 and 50 s show clear binding of chitotriose to the enzyme at a high level of detail. The success of this approach shows the potential for high-throughput drug screening and even structural enzymology on short timescales at bright synchrotron light sources.
Subject headings
- NATURVETENSKAP -- Kemi -- Materialkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Materials Chemistry (hsv//eng)
Keyword
- drug discovery
- protein structure
- X-ray crystallography
- serial crystallography
- time-resolved studies
- lysozyme
Publication and Content Type
- ref (subject category)
- art (subject category)
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