Sökning: id:"swepub:oai:DiVA.org:uu-347030" >
PDGF-A signaling is...
-
Gouveia, LeonorUppsala universitet,Vaskulärbiologi,Christer Betsholtz
(författare)
PDGF-A signaling is required for secondary alveolar septation and controls epithelial proliferation in the developing lung
- Artikel/kapitelEngelska2018
Förlag, utgivningsår, omfång ...
-
2018-04-10
-
The Company of Biologists,2018
-
printrdacarrier
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:uu-347030
-
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-347030URI
-
https://doi.org/10.1242/dev.161976DOI
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:138770924URI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
Platelet-derived growth factor A (PDGF-A) signaling through PDGF receptor a is essential for alveogenesis. Previous studies have shown that Pdgfa(-/-) mouse lungs have enlarged alveolar airspace with absence of secondary septation, both distinctive features of bronchopulmonary dysplasia. To study how PDGF-A signaling is involved in alveogenesis, we generated lung-specific Pdgfa knockout mice (Pdgfa(fl/-); Spc-cre) and characterized their phenotype postnatally. Histological differences between mutant mice and littermate controls were visible after the onset of alveogenesis and maintained until adulthood. Additionally, we generated Pdgfa(fl/-); Spc-cre; Pdgfra(GFP/+) mice in which Pdgfra(+) cells exhibit nuclear GFP expression. In the absence of PDGF-A, the number of Pdgfra(GFP+) cells was significantly decreased. In addition, proliferation of Pdgfra(GFP+) cells was reduced. During alveogenesis, Pdgfra(GFP+) myofibroblasts failed to form the alpha-smooth muscle actin rings necessary for alveolar secondary septation. These results indicate that PDGF-A signaling is involved in myofibroblast proliferation and migration. In addition, we show an increase in both the number and proliferation of alveolar type II cells in Pdgfa(fl/-); Spc-cre lungs, suggesting that the increased alveolar airspace is not caused solely by deficient myofibroblast function.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Betsholtz, ChristerKarolinska Institutet,Uppsala universitet,Vaskulärbiologi(Swepub:uu)chbet517
(författare)
-
Andrae, JohannaUppsala universitet,Vaskulärbiologi(Swepub:uu)johaandr
(författare)
-
Uppsala universitetVaskulärbiologi
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Development: The Company of Biologists145:70950-19911477-9129
Internetlänk
Hitta via bibliotek
Till lärosätets databas