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Multiplex plasma pr...
Multiplex plasma protein profiling identifies novel markers to discriminate patients with adenocarcinoma of the lung
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- Djureinovic, Dijana (author)
- Uppsala universitet,Klinisk och experimentell patologi,Patrick Micke
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- Pontén, Victor (author)
- Uppsala universitet,Klinisk och experimentell patologi,Patrick Micke
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- Landelius, Per (author)
- Uppsala universitet,Thoraxkirurgi
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- Al Sayegh, Sahar (author)
- Uppsala universitet,Vaskulärbiologi
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- Kappert, Kai (author)
- Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry; Center for Cardiovascular Research (CCR), Berlin, Germany
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- Kamali-Moghaddam, Masood (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Molekylära verktyg,Masood Kamali-Moghaddam
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- Micke, Patrick (author)
- Uppsala universitet,Klinisk och experimentell patologi,Patrick Micke
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- Ståhle, Elisabeth (author)
- Uppsala universitet,Thoraxkirurgi
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(creator_code:org_t)
- 2019-07-29
- 2019
- English.
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In: BMC Cancer. - : Springer Science and Business Media LLC. - 1471-2407. ; 19
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Abstract
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- Background:The overall prognosis of non-small cell lung cancer (NSCLC) is poor, and currently only patients with localized disease are potentially curable. Therefore, preferably non-invasively determined biomarkers that detect NSCLC patients at early stages of the disease are of high clinical relevance. The aim of this study was to identify and validate novel protein markers in plasma using the highly sensitive DNA-assisted multiplex proximity extension assay (PEA) to discriminate NSCLC from other lung diseases. Methods:Plasma samples were collected from a total of 343 patients who underwent surgical resection for different lung diseases, including 144 patients with lung adenocarcinoma (LAC),68 patients with non-malignant lung disease, 83 with lung metastasis of colorectal cancers and 48 patients with typical carcinoid. One microliter of plasma was analyzed using PEA, allowing detection and quantification of 92 established cancer related proteins. The concentrations of the plasma proteins were compared between disease groups.Results:The comparison between LAC and benign samples revealed significantly different plasma levels for four proteins; CXL17, CEACAM5, VEGFR2 and ERBB3 (adjusted p-value < 0.05). A multi-parameter classifier was developed to discriminate between samples from LAC patients and from patients with non-malignant lung conditions. With a bootstrap aggregated decision tree algorithm (TreeBagger) a sensitivity of 93% and specificity of 64% was achieved to detect LAC in this risk population. Conclusion:By applying the highly sensitive PEA, reliable protein profiles could be determined in microliter amounts of plasma. We further identified proteins that demonstrated different plasma concentration in defined disease groups and developed a signature that holds potential to be included in a screening assay for early lung cancer detection.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
Keyword
- lung cancer
- tumor markers
- blood
- serum
- screening
- biomarker
- Pathology
- Patologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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