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An advanced workflo...
An advanced workflow for single-particle imaging with the limited data at an X-ray free-electron laser
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- Assalauova, Dameli (författare)
- DESY, Notkestr 85, D-22607 Hamburg, Germany
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- Kim, Young Yong (författare)
- DESY, Notkestr 85, D-22607 Hamburg, Germany
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- Bobkov, Sergey (författare)
- Natl Res Ctr, Kurchatov Inst, Akad Kurchatova Pl 1, Moscow 123182, Russia
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- Khubbutdinov, Ruslan (författare)
- DESY, Notkestr 85, D-22607 Hamburg, Germany; Natl Res Nucl Univ MEPhI, Moscow Engn Phys Inst, Kashirskoe Sh 31, Moscow 115409, Russia
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- Rose, Max (författare)
- DESY, Notkestr 85, D-22607 Hamburg, Germany
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- Alvarez, Roberto (författare)
- Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA; Arizona State Univ, Sch Math & Stat Sci, Tempe, AZ 85287 USA
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- Andreasson, Jakob (författare)
- Acad Sci Czech Republ, ELI Beamlines, Inst Phys, CZ-18221 Prague, Czech Republic
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- Balaur, Eugeniu (författare)
- La Trobe Univ, Australian Res Council, La Trobe Inst Mol Sci LIMS, Dept Chem & Phys,Ctr Excellence Adv Mol Imaging, Melbourne, Vic 3086, Australia
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- Contreras, Alice (författare)
- Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA; Arizona State Univ, Biodesign Inst Ctr Immunotherapy Vaccines & Virot, Tempe, AZ 85287 USA
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- DeMirci, Hasan (författare)
- SLAC Natl Accelerator Lab, Stanford Pulse Inst, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA; Koc Univ, Dept Mol Biol & Genet, TR-34450 Istanbul, Turkey
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- Gelisio, Luca (författare)
- DESY, Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany
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- Hajdu, Janos (författare)
- Uppsala universitet,Molekylär biofysik,Acad Sci Czech Republ, ELI Beamlines, Inst Phys, CZ-18221 Prague, Czech Republic
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- Hunter, Mark S. (författare)
- SLAC Natl Accelerator Lab, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA
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- Kurta, Ruslan P. (författare)
- European XFEL, Holzkoppel 4, D-22869 Schenefeld, Germany
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- Li, Haoyuan (författare)
- SLAC Natl Accelerator Lab, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA; Stanford Univ, Phys Dept, 450 Jane Stanford Way, Stanford, CA 94305 USA
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- McFadden, Matthew (författare)
- Arizona State Univ, Biodesign Inst Ctr Immunotherapy Vaccines & Virot, Tempe, AZ 85287 USA
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- Nazari, Reza (författare)
- Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA; Arizona State Univ, Sch Engn Matter Transport & Energy, Tempe, AZ 85287 USA
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- Schwander, Peter (författare)
- Univ Wisconsin, Milwaukee, WI 53211 USA
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- Teslyuk, Anton (författare)
- Natl Res Ctr, Kurchatov Inst, Akad Kurchatova Pl 1, Moscow 123182, Russia; Moscow Inst Phys & Technol, Moscow 141700, Russia
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- Walter, Peter (författare)
- SLAC Natl Accelerator Lab, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA
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- Xavier, P. Lourdu (författare)
- DESY, Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany; SLAC Natl Accelerator Lab, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA; Max Planck Inst Struct & Dynam Matter, Luruper Chaussee 149, D-22761 Hamburg, Germany
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- Yoon, Chun Hong (författare)
- SLAC Natl Accelerator Lab, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA
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- Zaare, Sahba (författare)
- Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA; SLAC Natl Accelerator Lab, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA
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- Ilyin, Viacheslav A. (författare)
- Natl Res Ctr, Kurchatov Inst, Akad Kurchatova Pl 1, Moscow 123182, Russia; Moscow Inst Phys & Technol, Moscow 141700, Russia
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- Kirian, Richard A. (författare)
- Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA
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- Hogue, Brenda G. (författare)
- Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA; Arizona State Univ, Biodesign Inst Ctr Immunotherapy Vaccines & Virot, Tempe, AZ 85287 USA; Arizona State Univ, Ctr Appl Struct Discovery, Biodesign Inst, Tempe, AZ 85287 USA
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- Aquila, Andrew (författare)
- SLAC Natl Accelerator Lab, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA
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- Vartanyants, Ivan A. (författare)
- DESY, Notkestr 85, D-22607 Hamburg, Germany; Natl Res Nucl Univ MEPhI, Moscow Engn Phys Inst, Kashirskoe Sh 31, Moscow 115409, Russia
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(creator_code:org_t)
- 2020
- 2020
- Engelska.
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Ingår i: IUCrJ. - 2052-2525. ; 7, s. 1102-1113
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- An improved analysis for single-particle imaging (SPI) experiments, using the limited data, is presented here. Results are based on a study of bacteriophage PR772 performed at the Atomic, Molecular and Optical Science instrument at the Linac Coherent Light Source as part of the SPI initiative. Existing methods were modified to cope with the shortcomings of the experimental data: inaccessibility of information from half of the detector and a small fraction of single hits. The general SPI analysis workflow was upgraded with the expectation-maximization based classification of diffraction patterns and mode decomposition on the final virus-structure determination step. The presented processing pipeline allowed us to determine the 3D structure of bacteriophage PR772 without symmetry constraints with a spatial resolution of 6.9 nm. The obtained resolution was limited by the scattering intensity during the experiment and the relatively small number of single hits.
Ämnesord
- NATURVETENSKAP -- Kemi -- Materialkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Materials Chemistry (hsv//eng)
Nyckelord
- single-particle imaging
- three-dimensional virus reconstruction
- bacteriophage PR772
- XFELs
Publikations- och innehållstyp
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- art (ämneskategori)
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- Av författaren/redakt...
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Assalauova, Dame ...
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Kim, Young Yong
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Bobkov, Sergey
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Khubbutdinov, Ru ...
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Rose, Max
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Alvarez, Roberto
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visa fler...
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Andreasson, Jako ...
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Balaur, Eugeniu
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Contreras, Alice
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DeMirci, Hasan
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Gelisio, Luca
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Hajdu, Janos
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Hunter, Mark S.
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Kurta, Ruslan P.
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Li, Haoyuan
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McFadden, Matthe ...
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Nazari, Reza
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Schwander, Peter
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Teslyuk, Anton
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Walter, Peter
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Xavier, P. Lourd ...
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Yoon, Chun Hong
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Zaare, Sahba
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Ilyin, Viachesla ...
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Kirian, Richard ...
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Hogue, Brenda G.
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Aquila, Andrew
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Vartanyants, Iva ...
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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IUCrJ
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Uppsala universitet