WFRF:(Klimov V V)
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- Tikhonov, K.Russian Acad Sci, Inst Basic Biol Problems, Pushchino, Russia (author)
Quantification of bound bicarbonate in photosystem II#
- Article/chapterEnglish2018
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- Springer,2018
- printrdacarrier
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- LIBRIS-ID:oai:DiVA.org:uu-353182
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-353182URI
- https://doi.org/10.1007/s11099-017-0758-4DOI
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- Language:English
- Summary in:English
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- In this study, we presented a new approach for quantification of bicarbonate (HCO3-) molecules bound to PSII. Our method, which is based on a combination of membrane-inlet mass spectrometry (MIMS) and O-18-labelling, excludes the possibility of "non-accounted" HCO3- by avoiding (1) the employment of formate for removal of HCO3- from PSII, and (2) the extremely low concentrations of HCO3-/CO2 during online MIMS measurements. By equilibration of PSII sample to ambient CO2 concentration of dissolved CO2/HCO3-, the method ensures that all physiological binding sites are saturated before analysis. With this approach, we determined that in spinach PSII membrane fragments 1.1 +/- 0.1 HCO3- are bound per PSII reaction center, while none was bound to isolated PsbO protein. Our present results confirmed that PSII binds one HCO3- molecule as ligand to the non-heme iron of PSII, while unbound HCO3- optimizes the water-splitting reactions by acting as a mobile proton shuttle.
Subject headings and genre
- NATURVETENSKAP Biologi Biofysik hsv//swe
- NATURAL SCIENCES Biological Sciences Biophysics hsv//eng
- hydrogen carbonate
- inorganic carbon
- mass spectrometry
- Mn-stabilizing protein
- non-heme iron
- oxygen-evolving complex
Added entries (persons, corporate bodies, meetings, titles ...)
- Shevela, D.Umea Univ, Dept Chem, Chem Biol Ctr, Umea, Sweden (author)
- Klimov, V. V.Russian Acad Sci, Inst Basic Biol Problems, Pushchino, Russia (author)
- Messinger, Johannes,Prof. Dr.1963-Uppsala universitet,Molekylär biomimetik,Umea Univ, Dept Chem, Chem Biol Ctr, Umea, Sweden(Swepub:uu)johme269 (author)
- Russian Acad Sci, Inst Basic Biol Problems, Pushchino, RussiaUmea Univ, Dept Chem, Chem Biol Ctr, Umea, Sweden (creator_code:org_t)
Related titles
- In:Photosynthetica (Praha): Springer56:1, s. 210-2160300-36041573-9058
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