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Evaluation of the h...
Evaluation of the humoral immune response to a multicomponent recombinant vaccine against S-aureus in healthy pregnant heifers
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- Pujato, N. (författare)
- Univ Nacl Litoral, Fac Bioquim & Ciencias Biol, Santa Fe, Argentina
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- Camussone, C. M. (författare)
- Consejo Nacl Invest Cient & Tecn, Consejo Nacl Invest Cient & Tecn, Buenos Aires, DF, Argentina;INTA, Estn Expt Agr Rafaela, Santa Fe, Argentina
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- Renna, M. S. (författare)
- Consejo Nacl Invest Cient & Tecn, Consejo Nacl Invest Cient & Tecn, Buenos Aires, DF, Argentina;Univ Nacl Litoral, Fac Ciencias Vet, Esperanza, Santa Fe, Argentina
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visa fler...
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- Perrig, M. S. (författare)
- Univ Nacl Litoral, Fac Bioquim & Ciencias Biol, Santa Fe, Argentina;Consejo Nacl Invest Cient & Tecn, Consejo Nacl Invest Cient & Tecn, Buenos Aires, DF, Argentina
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- Morein, B. (författare)
- Uppsala universitet,Klinisk virologi
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- Calvinho, L. F. (författare)
- INTA, Estn Expt Agr Rafaela, Santa Fe, Argentina;Univ Nacl Litoral, Fac Ciencias Vet, Esperanza, Santa Fe, Argentina
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- Marcipar, I. S. (författare)
- Univ Nacl Litoral, Fac Bioquim & Ciencias Biol, Santa Fe, Argentina;Consejo Nacl Invest Cient & Tecn, Consejo Nacl Invest Cient & Tecn, Buenos Aires, DF, Argentina
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(creator_code:org_t)
- Elsevier, 2018
- 2018
- Engelska.
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Ingår i: The Veterinary Journal. - : Elsevier. - 1090-0233 .- 1532-2971. ; 235, s. 47-53
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Staphylococcus aureus is a worldwide pathogen that causes mastitis in dairy herds. Shortcomings in control programs have encouraged the development of vaccines against this pathogen. This study evaluated the vaccine candidate VacR, which included recombinant S. aureus protein clumping factor A (rClf), fibronectin binding protein A (rFnBP) and hemolysin beta (rBt), formulated with a novel immune-stimulating complex. Comparisons were made between healthy pregnant heifers that received either VacR (n = 8; VacR group) or phosphate buffered saline (PBS) plus adjuvant (control group) SC in the supramammary lymph node area on days 45 and 15 before the expected calving date. Blood and foremilk samples were collected from 7 to 60 days post-calving. After calving, heifers in the VacR group produced higher total IgG (IgG(total)) titers against each component, in both serum (rBt, 3.4 x 10(5); rClf, 3.1 x 10(5); rFnBP, 2.3 x 10(5)) and milk (rBt, 2.6 x 10(4); rClf, 1.3 x 10(4); rFnBP, 1.1 x 10(4)), than control heifers (P < 0.0001). There were increased concentrations of IgG(1), and IgG(2) in VacR group (P < 0.05), in both serum and milk. Humoral responses remained high throughout the period most susceptible to intramammary infections (P < 0.01). Antibodies produced against S. aureus rClf and rFnBP reduced bacterial adherence to fibronectin and fibrinogen by 73% and 67%, respectively (P < 0.001). Milk antibodies against these adhesins inhibited S. aureus invasion of a mammary epithelial cell line (MAC-T), resulting in 15.7% of bacteria internalized (P < 0.0001). There was an approximately 6-fold reduction in the hemolysis titer for the native hemolysin in the VacR group compared to the control group (P < 0.0001) and a significantly increase in the proportion of positive neutrophils (VacR, 29.7%; PBS, 13.1%) and the mean fluorescent index (VacR, 217.4; PBS, 152.6; P < 0.01) in the VacR group. The results suggest that VacR is a valuable vaccine candidate against S. aureus infections, and merits further field trials and experimental challenges. (C) 2018 Elsevier Ltd. All rights reserved.
Ämnesord
- LANTBRUKSVETENSKAPER -- Veterinärmedicin -- Annan veterinärmedicin (hsv//swe)
- AGRICULTURAL SCIENCES -- Veterinary Science -- Other Veterinary Science (hsv//eng)
Nyckelord
- Bovine mastitis
- Recombinant vaccine
- Staphylococcus aureus
- Vaccination of heifers
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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