Sökning: WFRF:(Ireland Michael J.)
> (2015-2019) >
Systematic approach...
Systematic approach for dissecting the molecular mechanisms of transcriptional regulation in bacteria
-
- Belliveau, Nathan M. (författare)
- CALTECH, Div Biol & Biol Engn, Pasadena, USA
-
- Barnes, Stephanie L. (författare)
- CALTECH, Div Biol & Biol Engn, Pasadena, USA
-
- Ireland, William T. (författare)
- CALTECH, Dept Phys, Pasadena, USA
-
visa fler...
-
- Jones, Daniel L. (författare)
- Uppsala universitet,Molekylär systembiologi,Science for Life Laboratory, SciLifeLab
-
- Sweredoski, Michael J. (författare)
- CALTECH, Beckman Inst, Proteome Explorat Lab, Pasadena, USA
-
- Moradian, Annie (författare)
- CALTECH, Beckman Inst, Proteome Explorat Lab, Pasadena, USA
-
- Hess, Sonja (författare)
- CALTECH, Beckman Inst, Proteome Explorat Lab, Pasadena, CA 91125 USA;MedImmune, Antibody Discovery & Prot Engn, Gaithersburg, USA
-
- Kinney, Justin B. (författare)
- Cold Spring Harbor Lab, Simons Ctr Quantitat Biol, POB 100, Cold Spring Harbor, USA
-
- Phillips, Rob (författare)
- CALTECH, Div Biol & Biol Engn, Pasadena, USA;CALTECH, Dept Phys, Pasadena, CA 91125 USA;CALTECH, Dept Appl Phys, Pasadena, CA 91125 USA
-
visa färre...
-
(creator_code:org_t)
- 2018-05-04
- 2018
- Engelska.
-
Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : NATL ACAD SCIENCES. - 0027-8424 .- 1091-6490. ; 115:21, s. E4796-E4805
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Gene regulation is one of the most ubiquitous processes in biology. However, while the catalog of bacterial genomes continues to expand rapidly, we remain ignorant about how almost all of the genes in these genomes are regulated. At present, characterizing the molecular mechanisms by which individual regulatory sequences operate requires focused efforts using low-throughput methods. Here, we take a first step toward multipromoter dissection and show how a combination of massively parallel reporter assays, mass spectrometry, and information-theoretic modeling can be used to dissect multiple bacterial promoters in a systematic way. We show this approach on both well-studied and previously uncharacterized promoters in the enteric bacterium Escherichia coli. In all cases, we recover nucleotide-resolution models of promoter mechanism. For some promoters, including previously unannotated ones, the approach allowed us to further extract quantitative biophysical models describing input-output relationships. Given the generality of the approach presented here, it opens up the possibility of quantitatively dissecting the mechanisms of promoter function in E. coli and a wide range of other bacteria.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- gene regulation
- massively parallel reporter assay
- quantitative models
- DNA affinity chromatography
- mass spectrometry
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas