Thromboinflammation as bioactivity assessment of H2O2-alkali modified titanium surfaces

Hulsart Billström, Gry,1982-Uppsala universitet,Ortopedi,Tillämpad materialvetenskap (author)

The release of growth factors from platelets, mediated by the coagulation and the complement system, plays an important role in the bone formation around implants. This study aimed at exploring the thromboinflammatory response of H2O2-alkali soaked commercially pure titanium grade 2 discs exposed to whole human blood, as a way to assess the bioactivity of the discs. Commercially pure titanium grade 2 discs were modified by soaking in H2O2, NaOH and Ca(OH)2. The platelet aggregation, coagulation activation and complement activation was assessed by exposing the discs to fresh whole blood from human donors. The platelet aggregation was examined by a cell counter and the coagulation and complement activation were assessed by ELISA-measurements of the concentration of thrombin-antithrombin complex, C3a and terminal complement complex. The modified surface showed a statistically significant increased platelet aggregation, coagulation activation and complement activation compared to unexposed blood. The surface also showed a statistically significant increase of coagulation activation compared to PVC. The results of this study showed that the H2O2-alkali soaked surfaces induced a thromboinflammatory response that indicates that the surfaces are bioactive.

Janson, OscarUppsala universitet,Tillämpad materialvetenskap(Swepub:uu)oscja567 (author)
Engqvist, Håkan,1972-Uppsala universitet,Tillämpad materialvetenskap,Nanoteknologi och funktionella material(Swepub:uu)hakaengq (author)
Welch, Ken,1968-Uppsala universitet,Nanoteknologi och funktionella material(Swepub:uu)kewel087 (author)
Hong, JaanUppsala universitet,Fasta tillståndets fysik,Klinisk immunologi(Swepub:uu)jaanhong (author)
Uppsala universitetOrtopedi (creator_code:org_t)

In:Journal of materials science. Materials in medicine: Springer Science and Business Media LLC30:60957-45301573-4838

By the author/editor: Hulsart Billströ ...; Janson, Oscar; Engqvist, Håkan, ...; Welch, Ken, 1968 ...; Hong, Jaan

About the subject

ENGINEERING AND TECHNOLOGY: ENGINEERING AND ...; and Materials Engine ...; and Other Materials ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Medical Biotechn ...; and Biomaterials Sci ...

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