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Amperometric Self-R...
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Campos-Beltran, DianaUniv Lubeck, Inst Expt & Clin Pharmacol & Toxicol, Ratzeburger Allee 160, D-23562 Lubeck, Germany
(author)
Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection
- Article/chapterEnglish2018
Publisher, publication year, extent ...
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2018-03-06
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MDPI,2018
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-358576
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-358576URI
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https://doi.org/10.3390/bios8010020DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:142640444URI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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D-serine is the major D-amino acid in the mammalian central nervous system. As the dominant co-agonist of the endogenous synaptic NMDA receptor, D-serine plays a role in synaptic plasticity, learning, and memory. Alterations in D-serine are linked to neuropsychiatric disorders including schizophrenia. Thus, it is of increasing interest to monitor the concentration of D-serine in vivo as a relevant player in dynamic neuron-glia network activity. Here we present a procedure for amperometric detection of D-serine with self-referencing ceramic-based microelectrode arrays (MEAs) coated with D-amino acid oxidase from the yeast Rhodotorula gracilis (RgDAAO). We demonstrate in vitro D-serine recordings with a mean sensitivity of 8.61 +/- 0.83 pA/mu M to D-serine, a limit of detection (LOD) of 0.17 +/- 0.01 mu M, and a selectivity ratio of 80:1 or greater for D-serine over ascorbic acid (mean +/- SEM; n = 12) that can be used for freely moving studies.
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Konradsson-Geuken, ÅsaUppsala universitet,Institutionen för farmaceutisk biovetenskap,Karolinska Inst, Dept Physiol & Pharmacol, S-17177 Stockholm, Sweden(Swepub:uu)asako336
(author)
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Quintero, Jorge E.Univ Kentucky, CenMeT, Lexington, KY 40506 USA;Quanteon LLC, Nicholasville, KY 40356 USA
(author)
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Marshall, LisaUniv Lubeck, Inst Expt & Clin Pharmacol & Toxicol, Ratzeburger Allee 160, D-23562 Lubeck, Germany
(author)
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Univ Lubeck, Inst Expt & Clin Pharmacol & Toxicol, Ratzeburger Allee 160, D-23562 Lubeck, GermanyInstitutionen för farmaceutisk biovetenskap
(creator_code:org_t)
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In:Biosensors: MDPI8:12079-6374
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