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Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae

Dumke, Roger (author)
Tech Univ Dresden, Dresden, Germany
Benitez, Alvaro J. (author)
Ctr Dis Control & Prevent, Atlanta, GA USA
Chalker, Victoria (author)
Publ Hlth England, London, England
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Gullsby, Karolina (author)
Uppsala universitet,Centrum för klinisk forskning, Gävleborg
Henrich, Birgit (author)
Univ Dusseldorf, Dusseldorf, Germany
Hidalgo-Grass, Carlos (author)
HGC Biomed Res Diagnost SL, Huelva, Spain
Hoogenboezem, Theo (author)
Sophia Childrens Univ Hosp, Erasmus MC Univ Med Ctr, Dept Pediat, Pediat Lab, Rotterdam, Netherlands
Kese, Darja (author)
Univ Ljubljana, Ljubljana, Slovenia
Loens, Katherine (author)
Univ Ziekenhuis Antwerpen, Edegem, Belgium
Maaskant, Jolanda (author)
Erasmus MC Univ Med Ctr, Virosci, Rotterdam, Netherlands
Michael-Gayego, Ayelet (author)
Hadassah Hebrew Univ, Med Ctr, Jerusalem, Israel
Moses, Allon E. (author)
Hadassah Hebrew Univ, Med Ctr, Jerusalem, Israel
Nir-Paz, Ran (author)
Hadassah Hebrew Univ, Med Ctr, Jerusalem, Israel
Pas, Suzan D. (author)
Erasmus MC Univ Med Ctr, Virosci, Rotterdam, Netherlands
Pereyre, Sabine (author)
Univ Bordeaux, Bordeaux, France
Petersen, Randi F. (author)
Statens Serum Inst, Copenhagen, Denmark
Rosenblatt, Meike (author)
Univ Dusseldorf, Dusseldorf, Germany
van Rossum, Annemarie M. C. (author)
Sophia Childrens Univ Hosp, Erasmus MC Univ Med Ctr, Dept Pediat, Div Infect Dis & Immunol, Rotterdam, Netherlands
Uldum, Soren A. (author)
Statens Serum Inst, Copenhagen, Denmark
Unger, Wendy W. J. (author)
Sophia Childrens Univ Hosp, Erasmus MC Univ Med Ctr, Dept Pediat, Pediat Lab, Rotterdam, Netherlands
Ursi, Dominique (author)
Univ Ziekenhuis Antwerpen, Edegem, Belgium
Winchell, Jonas M. (author)
Ctr Dis Control & Prevent, Atlanta, GA USA
Bebear, Cecile (author)
Univ Bordeaux, Bordeaux, France
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 (creator_code:org_t)
ELSEVIER SCIENCE INC, 2017
2017
English.
In: Diagnostic microbiology and infectious disease. - : ELSEVIER SCIENCE INC. - 0732-8893 .- 1879-0070. ; 88:2, s. 111-114
Related links:
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  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20 M. pneumoniae genomes per reaction.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Infectious Medicine (hsv//eng)

Keyword

Mytoplasma pneumoniae
Detection
Real-time PCR
Multi-center evaluation

Publication and Content Type

ref (subject category)
art (subject category)

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