Sökning: L773:0741 8329 OR L773:1873 6823 >
Early life stress a...
Early life stress and voluntary alcohol consumption in relation to Maoa methylation in male rats.
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- Bendre, Megha (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Centrum för klinisk forskning, Västerås,Comasco: Neuropsykofarmakologi,Uppsala Univ, Dept Neurosci, Sci Life Lab, Uppsala, Sweden.;Uppsala Univ, Ctr Clin Res, Vasteras, Sweden.
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- Granholm, Linnea (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Dept Pharmaceut Biosci Neuropharmacol Addict & Be, Uppsala, Sweden.
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- Drennan, Ryan (författare)
- EpigenDx Inc, Hopkinton, MA USA.
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- Meyer, Ann (författare)
- EpigenDx Inc, Hopkinton, MA USA.
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- Yan, Liying (författare)
- EpigenDx Inc, Hopkinton, MA USA.
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- Nilsson, Kent W. (författare)
- Uppsala universitet,Centrum för klinisk forskning, Västerås,Uppsala Univ, Ctr Clin Res, Vasteras, Sweden.
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- Nylander, Ingrid, 1957- (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Dept Pharmaceut Biosci Neuropharmacol Addict & Be, Uppsala, Sweden.
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- Comasco, Erika, 1982- (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Comasco: Neuropsykofarmakologi,Uppsala Univ, Dept Neurosci, Sci Life Lab, Uppsala, Sweden.
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(creator_code:org_t)
- Elsevier BV, 2019
- 2019
- Engelska.
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Ingår i: Alcohol. - : Elsevier BV. - 0741-8329 .- 1873-6823. ; 79, s. 7-16
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Early life stress (ELS) or alcohol consumption can influence DNA methylation and affect gene expression. The monoamine oxidase A (Maoa) encodes the enzyme that metabolizes monoaminergic neurotransmitters crucial for the stress response, alcohol reward, and reinforcement. Previously, we reported lower Maoa expression in the nucleus accumbens and dorsal striatum of male rats exposed to ELS during the first three postnatal weeks, and to voluntary alcohol consumption in adulthood, compared with controls. The present study continued to investigate the effect of ELS and alcohol consumption on Maoa methylation, and its relation to Maoa expression in these animals. We selected candidate CpGs after performing next-generation bisulfite sequencing of the Maoa promoter, intron 1-5, exons 5 and 6, together comprised of 107 CpGs, in a subgroup of rats. Pyrosequencing was used to analyse the methylation of ten candidate CpGs in the promoter and intron 1 in the entire sample. ELS and alcohol displayed an interaction effect on CpG-specific methylation in the dorsal striatum. CpG-specific methylation correlated with Maoa expression, corticosterone levels, and alcohol consumption in a brain region-specific manner. CpG-specific methylation in the Maoa promoter was a potential moderator of the interaction of ELS with alcohol consumption on Maoa expression in the NAc. However, the findings were sparse, did not survive correction for multiple testing, and the magnitude of differences in methylation levels was small. In conclusion, CpG-specific Maoa methylation in the promoter and intron 1 may associate with ELS, alcohol consumption and Maoa expression in reward-related brain regions.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Psykiatri (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Psychiatry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Nyckelord
- Alcohol
- DNA methylation
- Gene expression
- Maoa
- Maternal separation
- Stress
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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