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Claudin-3-deficient...
Claudin-3-deficient C57BL/6J mice display intact brain barriers
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- Dias, Mariana Castro (författare)
- Univ Bern, Theodor Kocher Inst, Bern, Switzerland
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- Coisne, Caroline (författare)
- Karolinska Institutet,Univ Bern, Theodor Kocher Inst, Bern, Switzerland
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- Lazarevic, Ivana (författare)
- Univ Bern, Theodor Kocher Inst, Bern, Switzerland
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- Baden, Pascale (författare)
- Univ Bern, Theodor Kocher Inst, Bern, Switzerland
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- Hata, Masaki (författare)
- Hyogo Coll Med, Lab Tumor Immunol & Cell Therapy, Nishinomiya, Hyogo, Japan
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- Iwamoto, Noriko (författare)
- Kobe Univ, Div Cell Biol, Grad Sch Med, Kobe, Hyogo, Japan
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- Francisco, David Miguel Ferreira (författare)
- Univ Bern, Interfac Bioinformat Unit, Bern, Switzerland;Univ Bern, Swiss Inst Bioinformat, Bern, Switzerland
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- Vanlandewijck, Michael (författare)
- AstraZeneca, Karolinska Inst, Cardio Metab Ctr, KI,AZ ICMC, Huddinge, Sweden
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- He, Liqun (författare)
- AstraZeneca, Karolinska Inst, Cardio Metab Ctr, KI,AZ ICMC, Huddinge, Sweden
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- Baier, Felix A. (författare)
- Univ Bern, Dept Biomed Res, Visceral Surg Res Lab, Bern, Switzerland
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- Stroka, Deborah (författare)
- Univ Bern, Dept Biomed Res, Visceral Surg Res Lab, Bern, Switzerland
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- Bruggmann, Remy (författare)
- Kobe Univ, Div Cell Biol, Grad Sch Med, Kobe, Hyogo, Japan;Univ Bern, Interfac Bioinformat Unit, Bern, Switzerland;Univ Bern, Swiss Inst Bioinformat, Bern, Switzerland
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- Lyck, Ruth (författare)
- Univ Bern, Theodor Kocher Inst, Bern, Switzerland
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- Enzmann, Gaby (författare)
- Univ Bern, Theodor Kocher Inst, Bern, Switzerland
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- Deutsch, Urban (författare)
- Univ Bern, Theodor Kocher Inst, Bern, Switzerland
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- Betsholtz, Christer (författare)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,AstraZeneca, Karolinska Inst, Cardio Metab Ctr, KI,AZ ICMC, Huddinge, Sweden
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- Furuse, Mikio (författare)
- Natl Inst Physiol Sci, Div Cell Struct, Okazaki, Aichi, Japan;Sch Life Sci, Dept Physiol Sci, Okazaki, Aichi, Japan
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- Tsukita, Shoichiro (författare)
- Univ Bern, Theodor Kocher Inst, Bern, Switzerland
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- Engelhardt, Britta (författare)
- Univ Bern, Theodor Kocher Inst, Bern, Switzerland
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(creator_code:org_t)
- 2019-01-18
- 2019
- Engelska.
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 9
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- The tight junction protein claudin-3 has been identified as a transcriptional target of the Wnt/beta-catenin signaling pathway regulating blood-brain barrier (BBB) maturation. In neurological disorders loss of claudin-3 immunostaining is observed at the compromised BBB and blood-cerebrospinal fluid barrier (BCSFB). Although these observations support a central role of claudin-3 in regulating brain barriers' tight junction integrity, expression of claudin-3 at the brain barriers has remained a matter of debate. This prompted us to establish claudin-3-/-C57BL/6J mice to study the role of claudin-3 in brain barrier integrity in health and neuroinflammation. Bulk and single cell RNA sequencing and direct comparative qRT-PCR analysis of brain microvascular samples from WT and claudin-3-/- mice show beyond doubt that brain endothelial cells do not express claudin-3 mRNA. Detection of claudin-3 protein at the BBB in vivo and in vitro is rather due to junctional reactivity of anti-claudin-3 antibodies to an unknown antigen still detected in claudin-3-/- brain endothelium. We confirm expression and junctional localization of claudin-3 at the BCSFB of the choroid plexus. Our study clarifies that claudin-3 is not expressed at the BBB and shows that absence of claudin-3 does not impair brain barrier function during health and neuroinflammation in C57BL/6J mice.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Dias, Mariana Ca ...
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Coisne, Caroline
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Lazarevic, Ivana
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Baden, Pascale
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Hata, Masaki
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Iwamoto, Noriko
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visa fler...
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Francisco, David ...
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Vanlandewijck, M ...
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He, Liqun
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Baier, Felix A.
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Stroka, Deborah
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Bruggmann, Remy
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Lyck, Ruth
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Enzmann, Gaby
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Deutsch, Urban
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Betsholtz, Chris ...
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Furuse, Mikio
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Tsukita, Shoichi ...
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Engelhardt, Brit ...
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Scientific Repor ...
- Av lärosätet
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Uppsala universitet
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Karolinska Institutet