Lipopolysaccharides at Solid and Liquid Interfaces: Models for Biophysical Studies of the Gram-negative Bacterial Outer Membrane

Paracini, NicolóMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV) (författare)

Lipopolysaccharides at Solid and Liquid Interfaces : Models for Biophysical Studies of the Gram-negative Bacterial Outer Membrane

Lipopolysaccharides (LPSs) are a constitutive element of the cell envelope of Gram-negative bacteria, representing the main lipid in the external leaflet of their outer membrane (OM) lipid bilayer. These unique surface-exposed glycolipids play a central role in the interactions of Gram-negative organisms with their surrounding environment and represent a key element for protection against antimicrobials and the development of antibiotic resistance. The biophysical investigation of a wide range of different types of in vitro model membranes containing reconstituted LPS has revealed functional and structural properties of these peculiar membrane lipids, providing molecular-level details of their interaction with antimicrobial compounds. LPS assemblies reconstituted at interfaces represent a versatile tool to study the properties of the Gram-negative OM by exploiting several surface-sensitive techniques, in particular X-ray and neutron scattering, which can probe the structure of thin films with sub-nanometer resolution. This review provides an overview of different approaches employed to investigate structural and biophysical properties of LPS, focusing on studies on Langmuir monolayers of LPS at the air/liquid interface and a range of supported LPS-containing model membranes reconstituted at solid/liquid interfaces.

Schneck, EmanuelPhysics Departent, Technische Universität Darmstadt, Darmstadt, Germany (författare)
Imberty, AnneUniversité Grenoble Alpes, CNRS, CERMAV, Grenoble, France (författare)
Micciulla, SamanthaInstitut Laue-Langevin, Grenoble, France (författare)
Malmö universitetInstitutionen för biomedicinsk vetenskap (BMV) (creator_code:org_t)

Ingår i:Advances in Colloid and Interface Science: Elsevier3010001-86861873-3727

Av författaren/redakt...: Paracini, Nicoló; Schneck, Emanuel; Imberty, Anne; Micciulla, Saman ...

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Mikrobiologi ino ...

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