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Sökning: id:"swepub:oai:DiVA.org:uu-378993" > Fine-Tuning of Sox1...

  • Corada, MonicaFIRC Inst Mol Oncol Fdn IFOM, Milan, Italy (författare)

Fine-Tuning of Sox17 and Canonical Wnt Coordinates the Permeability Properties of the Blood-Brain Barrier

  • Artikel/kapitelEngelska2019

Förlag, utgivningsår, omfång ...

  • 2019
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:uu-378993
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-378993URI
  • https://doi.org/10.1161/CIRCRESAHA.118.313316DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Rationale: The microvasculature of the central nervous system includes the blood-brain barrier (BBB), which regulates the permeability to nutrients and restricts the passage of toxic agents and inflammatory cells. Canonical Wnt/β-catenin signaling is responsible for the early phases of brain vascularization and BBB differentiation. However, this signal declines after birth, and other signaling pathways able to maintain barrier integrity at postnatal stage are still unknown.Objective: Sox17 (SRY [sex-determining region Y]-box 17) constitutes a major downstream target of Wnt/β-catenin in endothelial cells and regulates arterial differentiation. In the present article, we asked whether Sox17 may act downstream of Wnt/β-catenin in inducing BBB differentiation and maintenance.Methods and Results: Using reporter mice and nuclear staining of Sox17 and β-catenin, we report that although β-catenin signaling declines after birth, Sox17 activation increases and remains high in the adult. Endothelial-specific inactivation of Sox17 leads to increase of permeability of the brain microcirculation. The severity of this effect depends on the degree of BBB maturation: it is strong in the embryo and progressively declines after birth. In search of Sox17 mechanism of action, RNA sequencing analysis of gene expression of brain endothelial cells has identified members of the Wnt/β-catenin signaling pathway as downstream targets of Sox17. Consistently, we found that Sox17 is a positive inducer of Wnt/β-catenin signaling, and it acts in concert with this pathway to induce and maintain BBB properties. In vivo, inhibition of the β-catenin destruction complex or expression of a degradation-resistant β-catenin mutant, prevent the increase in permeability and retina vascular malformations observed in the absence of Sox17.Conclusions: Our data highlight a novel role for Sox17 in the induction and maintenance of the BBB, and they underline the strict reciprocal tuning of this transcription factor and Wnt/β-catenin pathway. Modulation of Sox17 activity may be relevant to control BBB permeability in pathological conditions.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Orsenigo, FabrizioFIRC Inst Mol Oncol Fdn IFOM, Milan, Italy (författare)
  • Bhat, Ganesh ParameshwarOsped San Raffaele, Mol Neurobiol Lab, Div Neurosci, Milan, Italy (författare)
  • Conze, Lei LiuUppsala universitet,Vaskulärbiologi(Swepub:uu)leliu102 (författare)
  • Breviario, FerruccioFIRC Inst Mol Oncol Fdn IFOM, Milan, Italy (författare)
  • Cunha, Sara I.Uppsala universitet,Vaskulärbiologi(Swepub:uu)sarcu762 (författare)
  • Claesson-Welsh, LenaUppsala universitet,Vaskulärbiologi(Swepub:uu)lcl04207 (författare)
  • Beznoussenko, Galina V.FIRC Inst Mol Oncol Fdn IFOM, Milan, Italy (författare)
  • Mironov, Alexander A.FIRC Inst Mol Oncol Fdn IFOM, Milan, Italy (författare)
  • Bacigaluppi, MarcoOsped San Raffaele, Neuroimmunol Unit, Div Neurosci, Inst Expt Neurol, Milan, Italy (författare)
  • Martino, GianvitoOsped San Raffaele, Neuroimmunol Unit, Div Neurosci, Inst Expt Neurol, Milan, Italy (författare)
  • Pitulescu, Mara E.Univ Munster, Max Planck Inst Mol Biomed, Dept Tissue Morphogenesis, Munster, Germany; Univ Munster, Fac Med, Munster, Germany (författare)
  • Adams, Ralf H.Univ Munster, Max Planck Inst Mol Biomed, Dept Tissue Morphogenesis, Munster, Germany; Univ Munster, Fac Med, Munster, Germany (författare)
  • Magnusson, PeetraUppsala universitet,Vaskulärbiologi(Swepub:uu)petrmagn (författare)
  • Dejana, ElisabettaUppsala universitet,Vaskulärbiologi,FIRC Inst Mol Oncol Fdn IFOM, Milan, Italy; Univ Milan, Dept Oncol & Hematooncol, Milan, Italy(Swepub:uu)elide443 (författare)
  • FIRC Inst Mol Oncol Fdn IFOM, Milan, ItalyOsped San Raffaele, Mol Neurobiol Lab, Div Neurosci, Milan, Italy (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Circulation Research124:4, s. 511-5250009-73301524-4571

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