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An Unexpected Role ...
An Unexpected Role of Hyaluronic Acid in Trafficking siRNA Across the Cellular Barrier : The First Biomimetic, Anionic, Non-Viral Transfection Method
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- Paidikondala, Maruthibabu, 1985- (author)
- Uppsala universitet,Polymerkemi
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- Rangasami, Vignesh Kumar (author)
- Tampere Univ, Fac Med & Hlth Technol, Bioengn & Nanomed Lab, Korkeakoulunkatu 3, Tampere 33720, Finland;Tampere Univ, BioMediTech Inst, Korkeakoulunkatu 3, Tampere 33720, Finland
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- Nawale, Ganesh N. (author)
- Uppsala universitet,Polymerkemi
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- Casalini, Tommaso (author)
- SUPSI, Dept Innovat Technol, Inst Mech Engn & Mat Engn, CH-6928 Manno, Switzerland;Swiss Fed Inst Technol, Dept Chem & Appl Biosci, Inst Chem & Bioengn, CH-8093 Zurich, Switzerland
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- Perale, Giuseppe (author)
- SUPSI, Dept Innovat Technol, Inst Mech Engn & Mat Engn, CH-6928 Manno, Switzerland
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- Kadekar, Sandeep (author)
- Uppsala universitet,Polymerkemi
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- Mohanty, Gaurav (author)
- Tampere Univ, Fac Engn & Nat Sci, Mat Sci & Environm Engn, Tampere, Finland
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- Salminen, Turkka (author)
- Tampere Univ, Tampere Microscopy Ctr, Tampere, Finland
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- Oommen, Oommen P. (author)
- Tampere Univ, Fac Med & Hlth Technol, Bioengn & Nanomed Lab, Korkeakoulunkatu 3, Tampere 33720, Finland;Tampere Univ, BioMediTech Inst, Korkeakoulunkatu 3, Tampere 33720, Finland
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- Varghese, Oommen P., 1977- (author)
- Uppsala universitet,Polymerkemi
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(creator_code:org_t)
- WILEY-V C H VERLAG GMBH, 2019
- 2019
- English.
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In: Angewandte Chemie International Edition. - : WILEY-V C H VERLAG GMBH. - 1433-7851 .- 1521-3773. ; 58:9, s. 2815-2819
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Circulating nucleic acids, such as short interfering RNA (siRNA), regulate many biological processes; however, the mechanism by which these molecules enter the cell is poorly understood. The role of extracellular-matrix-derived polymers in binding siRNAs and trafficking them across the plasma membrane is reported. Thermal melting, dynamic light scattering, scanning electron microscopy, and computational analysis indicate that hyaluronic acid can stabilize siRNA via hydrogen bonding and Van der Waals interactions. This stabilization facilitated HA size- and concentration-dependent gene silencing in a CD44-positive human osteosarcoma cell line (MG-63) and in human mesenchymal stromal cells (hMSCs). This native HA-based siRNA transfection represents the first report on an anionic, non-viral delivery method that resulted in approximately 60% gene knockdown in both cell types tested, which correlated with a reduction in translation levels.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- nanoparticles
- extracellular matrix
- hyaluronic acid
- RNAi
- transfection
Publication and Content Type
- ref (subject category)
- art (subject category)
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Paidikondala, Ma ...
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Rangasami, Vigne ...
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Nawale, Ganesh N ...
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Casalini, Tommas ...
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Perale, Giuseppe
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Kadekar, Sandeep
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Mohanty, Gaurav
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Salminen, Turkka
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Oommen, Oommen P ...
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Varghese, Oommen ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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Angewandte Chemi ...
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Uppsala University