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Search: id:"swepub:oai:DiVA.org:uu-382822" > Luminescent CeO2:Eu...

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  • Henning, Dorian F.Karolinska Inst, Dept Microbiol Tumor & Cell Biol, SE-17177 Stockholm, Sweden;Imperial Coll London, Dept Comp, London, England (author)

Luminescent CeO2:Eu3+ nanocrystals for robust in situ H2O2 real-time detection in bacterial cell cultures

  • Article/chapterEnglish2019

Publisher, publication year, extent ...

  • ELSEVIER ADVANCED TECHNOLOGY,2019
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  • LIBRIS-ID:oai:DiVA.org:uu-382822
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-382822URI
  • https://doi.org/10.1016/j.bios.2019.03.012DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:140710377URI

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  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Hydrogen peroxide (H2O2) quantification in biomedicine is valuable as inflammation biomarker but also in assays employing enzymes that generate or consume H2O2 linked to a specific biomarker. Optical H2O2 detection is typically performed through peroxidase-coupled reactions utilizing organic dyes that suffer, however, from poor stability/reproducibility and also cannot be employed in situ in dynamic complex cell cultures to monitor H2O2 levels in real-time. Here, we utilize enzyme-mimetic CeO2 nanocrystals that are sensitive to H2O2 and study the effect of H2O2 presence on their electronic and luminescent properties. We produce and dope with Eu3+ these particles in a single-step by flame synthesis and directly deposit them on Si and glass substrates to fabricate nanoparticle layers to monitor in real-time and in situ the H2O2 concentrations generated by Streptococcus pneumoniae clinical isolates. Furthermore, the small CeO2:Eu3+ nanocrystals are combined in a single-step with larger, non-responsive Y2O3:Tb3+ nanoparticles during their double-nozzle flame synthesis to engineer hybrid luminescent nanoaggregates as ratiometric robust biosensors. We demonstrate the functionality of these biosensors by monitoring their response in the presence of a broad range of H2O2 concentrations in vitro from S. pneumoniae, highlighting their potential for facile real-time H2O2 detection in vitro in cell cultures.

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  • Merkl, PadrykKarolinska Institutet (author)
  • Yun, ChanghunKarolinska Inst, Dept Microbiol Tumor & Cell Biol, SE-17177 Stockholm, Sweden (author)
  • Iovino, FedericoKarolinska Institutet (author)
  • Xie, Ling,1982-Uppsala universitet,Tillämpad materialvetenskap(Swepub:uu)xieli203 (author)
  • Mouzourakis, EleftheriosUniv Ioannina, Dept Phys, GR-45110 Ioannina, Greece (author)
  • Moularas, ConstantinosUniv Ioannina, Dept Phys, GR-45110 Ioannina, Greece (author)
  • Deligiannakis, YiannisUniv Ioannina, Dept Phys, GR-45110 Ioannina, Greece (author)
  • Henriques-Normark, BirgittaKarolinska Institutet (author)
  • Leifer, Klaus,1965-Uppsala universitet,Tillämpad materialvetenskap(Swepub:uu)kllei839 (author)
  • Sotiriou, Georgios A.Karolinska Institutet (author)
  • Karolinska InstitutetKarolinska Inst, Dept Microbiol Tumor & Cell Biol, SE-17177 Stockholm, Sweden;Imperial Coll London, Dept Comp, London, England (creator_code:org_t)

Related titles

  • In:Biosensors & bioelectronics: ELSEVIER ADVANCED TECHNOLOGY132, s. 286-2930956-56631873-4235

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