Sökning: WFRF:(Ahmed Qamar Uddin) >
Optimization of Hyp...
Optimization of Hyperglycemic Induction in Zebrafish and Evaluation of Its Blood Glucose Level and Metabolite Fingerprint Treated with Psychotria malayana Jack Leaf Extract
-
- Benchoula, Khaled (författare)
- Int Islamic Univ Malaysia, Kulliyyah Pharm, Dept Basic Med Sci, Kuantan 25200, Pahang, Malaysia
-
- Khatib, Alfi (författare)
- Int Islamic Univ Malaysia, Kulliyyah Pharm, Dept Pharmaceut Chem, Kuantan 25200, Pahang, Malaysia
-
- Quzwain, Fairuz M. C. (författare)
- Univ Jambi, Fac Med, Jambi 36122, Indonesia
-
visa fler...
-
- Mohamad, Che Anuar Che (författare)
- Int Islamic Univ Malaysia, Kulliyyah Pharm, Dept Basic Med Sci, Kuantan 25200, Pahang, Malaysia
-
- Sulaiman, Wan Mohd Azizi Wan (författare)
- Int Islamic Univ Malaysia, Kulliyyah Pharm, Dept Basic Med Sci, Kuantan 25200, Pahang, Malaysia
-
- Wahab, Ridhwan Abdul (författare)
- Int Islamic Univ Malaysia, Dept Biomed Sci, Kulliyyah Allied Hlth Sci, Kuantan 25200, Pahang, Malaysia
-
- Ahmed, Qamar Uddin (författare)
- Int Islamic Univ Malaysia, Kulliyyah Pharm, Dept Pharmaceut Chem, Kuantan 25200, Pahang, Malaysia
-
- Ghaffar, Majid Abdul (författare)
- Int Islamic Univ Malaysia, Kulliyyah Pharm, Dept Pharmaceut Chem, Kuantan 25200, Pahang, Malaysia
-
- Saiman, Mohd Zuwairi (författare)
- Univ Malaya, Inst Biol Sci, Fac Sci, Kuala Lumpur 50603, Malaysia
-
- Alajmi, Mohamed F. (författare)
- King Saud Univ, Dept Pharmacognosy, Coll Pharm, Riyadh 11451, Saudi Arabia
-
- El-Seedi, Hesham (författare)
- Uppsala universitet,Farmakognosi
-
visa färre...
-
(creator_code:org_t)
- 2019-04-17
- 2019
- Engelska.
-
Ingår i: Molecules. - : MDPI. - 1431-5157 .- 1420-3049. ; 24:8
- Relaterad länk:
-
https://doi.org/10.3...
-
visa fler...
-
https://uu.diva-port... (primary) (Raw object)
-
https://www.mdpi.com...
-
https://urn.kb.se/re...
-
https://doi.org/10.3...
-
visa färre...
Abstract
Ämnesord
Stäng
- A standard protocol to develop type 1 diabetes in zebrafish is still uncertain due to unpredictable factors. In this study, an optimized protocol was developed and used to evaluate the anti-diabetic activity of Psychotria malayana leaf. The aims of this study were to develop a type 1 diabetic adult zebrafish model and to evaluate the anti-diabetic activity of the plant extract on the developed model. The ability of streptozotocin and alloxan at a different dose to elevate the blood glucose levels in zebrafish was evaluated. While the anti-diabetic activity of P. malayana aqueous extract was evaluated through analysis of blood glucose and LC-MS analysis fingerprinting. The results indicated that a single intraperitoneal injection of 300 mg/kg alloxan was the optimal dose to elevate the fasting blood glucose in zebrafish. Furthermore, the plant extract at 1, 2, and 3 g/kg significantly reduced blood glucose levels in the diabetic zebrafish. In addition, LC-MS-based fingerprinting indicated that 3 g/kg plant extract more effective than other doses. Phytosterols, sugar alcohols, sugar acid, free fatty acids, cyclitols, phenolics, and alkaloid were detected in the extract using GC-MS. In conclusion, P. malayana leaf aqueous extract showed anti-diabetic activity on the developed type 1 diabetic zebrafish model.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Type 1 diabetes
- zebrafish
- Psychotria malayana
- alloxan
- insulin
- streptozotocin
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Benchoula, Khale ...
-
Khatib, Alfi
-
Quzwain, Fairuz ...
-
Mohamad, Che Anu ...
-
Sulaiman, Wan Mo ...
-
Wahab, Ridhwan A ...
-
visa fler...
-
Ahmed, Qamar Udd ...
-
Ghaffar, Majid A ...
-
Saiman, Mohd Zuw ...
-
Alajmi, Mohamed ...
-
El-Seedi, Hesham
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
- Artiklar i publikationen
-
Molecules
- Av lärosätet
-
Uppsala universitet