Search: (WFRF:(Mandava Chandra Sekhar 1978 )) >
The mechanism of er...
The mechanism of error induction by the antibiotic viomycin provides insight into the fidelity mechanism of translation
-
- Holm, Mikael, 1984- (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
-
- Mandava, Chandra Sekhar, 1978- (author)
- Uppsala universitet,Molekylärbiologi
-
- Ehrenberg, Måns (author)
- Uppsala universitet,Molekylärbiologi
-
show more...
-
- Sanyal, Suparna (author)
- Uppsala universitet,Molekylärbiologi
-
show less...
-
(creator_code:org_t)
- ELIFE SCIENCES PUBLICATIONS LTD, 2019
- 2019
- English.
-
In: eLIFE. - : ELIFE SCIENCES PUBLICATIONS LTD. - 2050-084X. ; 8
- Related links:
-
https://doi.org/10.7...
-
show more...
-
https://uu.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.7...
-
show less...
Abstract
Subject headings
Close
- Applying pre-steady state kinetics to an Escherichia-coli-based reconstituted translation system, we have studied how the antibiotic viomycin affects the accuracy of genetic code reading. We find that viomycin binds to translating ribosomes associated with a ternary complex (TC) consisting of elongation factor Tu (EF-Tu), aminoacyl tRNA and GTP, and locks the otherwise dynamically flipping monitoring bases A1492 and A1493 into their active conformation. This effectively prevents dissociation of near- and non-cognate TCs from the ribosome, thereby enhancing errors in initial selection. Moreover, viomycin shuts down proofreading-based error correction. Our results imply a mechanism in which the accuracy of initial selection is achieved by larger backward rate constants toward TC dissociation rather than by a smaller rate constant for GTP hydrolysis for near- and non-cognate TCs. Additionally, our results demonstrate that translocation inhibition, rather than error induction, is the major cause of cell growth inhibition by viomycin.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
eLIFE
(Search for host publication in LIBRIS)
To the university's database