Mitochondrial function and membrane integrity: an in vitro comparison between six commonly used opioids

Nylander, Erik,1986-Uppsala universitet,Institutionen för farmaceutisk biovetenskap (författare)

Introduction: There is an ongoing opioid crisis in the United States where the illicit and non-medical use of prescription opioids is associated with an increasing number of overdose deaths. Few studies have investigated the effects of opioid-induced effects on cell viability, and comparative studies are scarce. Here we examine the toxic effect on cell viability from six commonly used opioids; methadone, morphine, oxycodone, hydromorphone, ketobemidone, and fentanyl with respect to mitochondrial and membrane function in vitro. Methods: The opioids were tested in four different cell cultures; primary cortical cell cultures, human neuroblastoma SH-SY5Y cells, and both differentiated and undifferentiated neuroblastoma/glioma hybrid NG108-15 cells. Results: The six different opioids displayed the same trend of reduced cell viability in all four cell cultures. The ranking of opioids, with respect to reduced cell viability were as follows; methadone, fentanyl, ketobemidone, oxycodone, hydromorphone, and morphine. Conclusion: Methadone was ranked as the most toxic opioid closely followed by fentanyl. Ketobemidone and oxycodone had modest effects while both hydromorphone and morphine only displayed little to no negative impact on cell viability.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine hsv//eng
Methadone
Morphine
Oxycodone
Hydromorphone
Ketobemidone
Fentanyl
Cell viability
primary cell cultures
NG108-15
SH-SY5Y
Opioids
Farmaceutisk vetenskap
Pharmaceutical Science

Katila, LenkaUppsala universitet,Anestesiologi och intensivvård(Swepub:uu)lenka900 (författare)
Zelleroth, Sofia,1990-Uppsala universitet,Institutionen för farmaceutisk biovetenskap(Swepub:uu)sofze624 (författare)
Birgersson, Jennifer (författare)
Nyberg, Fred,1945-Uppsala universitet,Avdelningen för biologisk beroendeforskning(Swepub:uu)frednybe (författare)
Gröndbladh, Alfhild,1983-Uppsala universitet,Institutionen för farmaceutisk biovetenskap(Swepub:uu)alfgr942 (författare)
Hallberg, M,1971-Uppsala universitet,Avdelningen för biologisk beroendeforskning(Swepub:uu)mha03066 (författare)
Uppsala universitetInstitutionen för farmaceutisk biovetenskap (creator_code:org_t)

Av författaren/redakt...: Nylander, Erik, ...; Katila, Lenka; Zelleroth, Sofia ...; Birgersson, Jenn ...; Nyberg, Fred, 19 ...; Gröndbladh, Alfh ...; visa fler...; Hallberg, M, 197 ...; visa färre...

Om ämnet

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.