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  • Pfeifer, H.Goethe Univ, Dept Med, Hematol Oncol, Frankfurt, Germany (author)

Standardisation and consensus guidelines for minimal residual disease assessment in Philadelphia-positive acute lymphoblastic leukemia (Ph plus ALL) by real-time quantitative reverse transcriptase PCR of e1a2 BCR-ABL1

  • Article/chapterEnglish2019

Publisher, publication year, extent ...

  • 2019-03-11
  • NATURE PUBLISHING GROUP,2019
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-393341
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-393341URI
  • https://doi.org/10.1038/s41375-019-0413-0DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Minimal residual disease (MRD) is a powerful prognostic factor in acute lymphoblastic leukemia (ALL) and is used for patient stratification and treatment decisions, but its precise role in Philadelphia chromosome positive ALL is less clear. This uncertainty results largely from methodological differences relating to the use of real-time quantitative PCR (qRT-PCR) to measure BCR-ABL1 transcript levels for MRD analysis. We here describe the first results by the EURO-MRD consortium on standardization of qRT-PCR for the e1a2 BCR-ABL1 transcript in Ph + ALL, designed to overcome the lack of standardisation of laboratory procedures and data interpretation. Standardised use of EAC primer/probe sets and of centrally prepared plasmid standards had the greatest impact on reducing interlaboratory variability. In QC1 the proportion of analyses with BCR-ABL1/ABL1 ratios within half a log difference were 40/67 (60%) and 52/67 (78%) at 10(-3) and 36/67 (53%) and 53/67 (79%) at 10(-4)BCR-ABL1/ABL1. Standardized RNA extraction, cDNA synthesis and cycler platforms did not improve results further, whereas stringent application of technical criteria for assay quality and uniform criteria for data interpretation and reporting were essential. We provide detailed laboratory recommendations for the standardized MRD analysis in routine diagnostic settings and in multicenter clinical trials for Ph + ALL.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Cazzaniga, G.Univ Milano Bicocca, San Gerardo Hosp, Pediat Clin, Monza, Italy (author)
  • van der Velden, V. H. J.Erasmus MC, Dept Immunol, Med Ctr Rotterdam, Rotterdam, Netherlands (author)
  • Cayuele, J. M.Univ Paris Diderot, Univ Hosp St Louis, Hematol Lab, Paris, France;Univ Paris Diderot, Univ Hosp St Louis, EA3518, Paris, France (author)
  • Schafer, B.Kinderspital Zurich, Onkol Diagnost Lab, Zurich, Switzerland (author)
  • Spinelli, O.ASST Papa Giovanni XXIII, Hematol & Bone Marrow Transplant Unit, Bergamo, Italy (author)
  • Akiki, S.Birmingham Womens NHS Fdn Trust, Reg Genet Lab, Birmingham, W Midlands, England (author)
  • Avigad, S.Schneider Childrens Med Ctr Israel, Pediat Hematol Oncol, Petah Tiqwa, Israel (author)
  • Bendit, IUniv Sao Paulo, Med Sch, Div Hematol & Hemotherapy, Sao Paulo, Brazil (author)
  • Borg, K.Inst Hematol & Transfus Med, Dept Diagnost Hematol, Warsaw, Poland (author)
  • Cave, H.Hop Robert Debre, Dept Genet Biochem, Paris, France (author)
  • Elia, L.Univ Roma La Sapienza, Dept Hematol, Lab Mol Biol, Rome, Italy (author)
  • Reshmi, S. C.Nationwide Childrens Hosp, Inst Genom Med, Columbus, OH USA;Ohio State Univ, Columbus, OH USA (author)
  • Gerrard, G.Imperial Coll London, Ctr Hematol, Imperial Mol Pathol, London, England (author)
  • Hayette, S.Hosp Civils Lyon, Ctr Hosp Lyon Sud, Lab Mol Biol, Lyon, France;Hosp Civils Lyon, Ctr Hosp Lyon Sud, UMR5239, Lyon, France (author)
  • Hermansson, MonicaUppsala universitet,Medicinsk genetik och genomik(Swepub:uu)moniherm (author)
  • Juh, A.Natl Univ Singapore Hosp, Dept Pediat, Div Pediat Hematol Oncol, Singapore, Singapore (author)
  • Jurcek, T.Masaryk Univ, Dept Internal Med Hematol & Oncol, Brno, Czech Republic;Univ Hosp Brno, Brno, Czech Republic (author)
  • Chillon, M. C.Salamanca Univ, CSIC, Univ Hosp Salamanca IBSAL, Dept Hematol, Salamanca, Spain;Salamanca Univ, Canc Res Ctr, IBMCC, CSIC, Salamanca, Spain (author)
  • Homburg, C.Sanquin Diagnost, Dept Expt Immunhematol Immuncytol, Amsterdam, Netherlands (author)
  • Martinelli, G.Univ Bologna, Dept Expt Diagnost & Specialty Med, Bologna, Italy (author)
  • Kairisto, VTurku Univ Hosp, TYKSLAB, Lab Mol Genet, Turku, Finland (author)
  • Langen, T.Univ Leipzig, Abt Hamatol & Internist Onkol, Leipzig, Germany (author)
  • Lion, T.Labdia Labordiagnost & Med Univ, Childrens Canc Res Inst, Vienna, Austria (author)
  • Mueller, M. C.Med Univ Heidelberg, Med Klin 3, Fak Mannheim, Mannheim, Germany (author)
  • Pane, F.Azienda Osped Univ Federico II Napoli Univ, Naples, Italy (author)
  • Rai, L.UCL, Dept Hematol, Royal Free Campus, London, England (author)
  • Damm-Welk, C.Justus Liebig Univ Giessen, Univ Hosp, Dept Pediat Hematol & Oncol, Giessen, Germany (author)
  • Sacha, T.Jagiellonain Univ Krakow, Hematol Dept, Krakow, Poland (author)
  • Schnittger, S.MLL, Max Lebsche Pl 31, Munich, Germany (author)
  • Touloumenidou, T.G Papanicolaou Hosp, Hematol Dept, Thessaloniki, Greece;G Papanicolaou Hosp, HCT Unit, Thessaloniki, Greece (author)
  • Valerhaugen, H.Oslo Univ Hosp, Norwegian Radium Hosp, Dept Pathol, Oslo, Norway (author)
  • Vandenberghe, P.Univ Hosp Leuven, Ctr Human Genet, Leuven, Belgium;Katholieke Univ Leuven, Leuven, Belgium (author)
  • Zuna, J.Charles Univ Prague, CLIP, Dept Paediat Haematol & Oncol, Fac Med 2, Prague, Czech Republic;UH Motol, Prague, Czech Republic (author)
  • Server, H.Goethe Univ, Dept Med, Hematol Oncol, Frankfurt, Germany (author)
  • Herrmann, E.Goethe Univ, Inst Biostat & Math Modellierung, Frankfurt, Germany (author)
  • Markovic, S.Goethe Univ, Dept Med, Hematol Oncol, Frankfurt, Germany (author)
  • van Dongen, J. J. M.Erasmus MC, Dept Immunol, Med Ctr Rotterdam, Rotterdam, Netherlands;Leiden Univ, Dept Immunohematol & Blood Transfus, Med Ctr, Leiden, Netherlands (author)
  • Ottmann, O. G.Cardiff Univ, Sch Med, Div Canc & Hematol, Cardiff, S Glam, Wales (author)
  • Goethe Univ, Dept Med, Hematol Oncol, Frankfurt, GermanyUniv Milano Bicocca, San Gerardo Hosp, Pediat Clin, Monza, Italy (creator_code:org_t)

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