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Monitoring of Prote...
Monitoring of Protein Biomarkers of Inflammation in Human Traumatic Brain Injury Using Microdialysis and Proximity Extension Assay Technology in Neurointensive Care
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- Dyhrfort, Philip (author)
- Uppsala universitet,Enblad: Neurokirurgi
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- Shen, Qiujin (author)
- Uppsala universitet,Molekylära verktyg
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- Clausen, Fredrik, 1973- (author)
- Uppsala universitet,Institutionen för neurovetenskap,Uppsala Univ, Dept Neurosci, Sect Neurosurg, Uppsala, Sweden,Enblad:neurokirurgi
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- Thulin, Måns, 1986- (author)
- Uppsala universitet,Statistiska institutionen,Univ Edinburgh, Sch Math, Edinburgh, Midlothian, Scotland;Univ Edinburgh, Maxwell Inst Math Sci, Edinburgh, Midlothian, Scotland
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- Enblad, Per (author)
- Uppsala universitet,Enblad: Neurokirurgi
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- Kamali-Moghaddam, Masood (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi
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- Lewén, Anders, 1965- (author)
- Uppsala universitet,Enblad: Neurokirurgi
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- Hillered, Lars, 1952- (author)
- Uppsala universitet,Institutionen för neurovetenskap
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(creator_code:org_t)
- MARY ANN LIEBERT, INC, 2019
- 2019
- English.
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In: Journal of Neurotrauma. - : MARY ANN LIEBERT, INC. - 0897-7151 .- 1557-9042. ; 36:20, s. 2872-2885
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https://uu.diva-port... (primary) (Raw object)
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Abstract
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- Traumatic brain injury (TBI) is followed by secondary injury mechanisms strongly involving neuroinflammation. To monitor the complex inflammatory cascade in human TBI, we used cerebral microdialysis (MD) and multiplex proximity extension assay (PEA) technology and simultaneously measured levels of 92 protein biomarkers of inflammation in MD samples every three hours for five days in 10 patients with severe TBI under neurointensive care. One mu L MD samples were incubated with paired oligonucleotide-conjugated antibodies binding to each protein, allowing quantification by real-time quantitative polymerase chain reaction. Sixty-nine proteins were suitable for statistical analysis. We found five different patterns with either early (<48 h; e.g., CCL20, IL6, LIF, CCL3), mid (48-96 h; e.g., CCL19, CXCL5, CXCL10, MMP1), late (>96 h; e.g., CD40, MCP2, MCP3), biphasic peaks (e.g., CXCL1, CXCL5, IL8) or stable (e.g., CCL4, DNER, VEGFA)/low trends. High protein levels were observed for e.g., CXCL1, CXCL10, MCP1, MCP2, IL8, while e.g., CCL28 and MCP4 were detected at low levels. Several proteins (CCL8, -19, -20, -23, CXCL1, -5, -6, -9, -11, CST5, DNER, Flt3L, and SIRT2) have not been studied previously in human TBI. Cross-correlation analysis revealed that LIF and CXCL5 may play a central role in the inflammatory cascade. This study provides a unique data set with individual temporal trends for potential inflammatory biomarkers in patients with TBI. We conclude that the combination of MD and PEA is a powerful tool to map the complex inflammatory cascade in the injured human brain. The technique offers new possibilities of protein profiling of complex secondary injury pathways.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
Keyword
- biomarkers
- inflammation
- microdialysis
- molecular tools
- neurointensive care
- proteomics
- traumatic brain injury
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Dyhrfort, Philip
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Shen, Qiujin
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Clausen, Fredrik ...
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Thulin, Måns, 19 ...
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Enblad, Per
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Kamali-Moghaddam ...
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Lewén, Anders, 1 ...
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Hillered, Lars, ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Neurology
- Articles in the publication
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Journal of Neuro ...
- By the university
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Uppsala University