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Optimisation of the...
Optimisation of the droplet split design for high acoustic particle enrichment in droplet microfluidics
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- Fornell, Anna (författare)
- Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,Embla
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- Liu, Zhenhua, 1992- (författare)
- Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,Embla
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- Tenje, Maria (författare)
- Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,Embla
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(creator_code:org_t)
- Elsevier BV, 2020
- 2020
- Engelska.
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Ingår i: Microelectronic Engineering. - : Elsevier BV. - 0167-9317 .- 1873-5568. ; 226
- Relaterad länk:
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https://urn.kb.se/re...
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visa fler...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We have characterised three droplet split designs for acoustic particle enrichment in water-in-oil droplets. The microfluidic channel design included a droplet generation junction, acoustic focusing channel and a trident-shaped droplet split. The microfluidic channels were dry-etched in silicon and sealed with glass lids by anodic bonding. To each microfluidic chip a piezoelectric transducer was glued, and at actuation of the transducer at the fundamental resonance frequency of the acoustic focusing channel (1.91–1.93 MHz), a half wavelength standing wave field was created between the channel walls. The acoustic force focused the encapsulated particles (3.2 μm, 4.8 μm and 9.9 μm diameter polystyrene microbeads) to the centre-line of the droplets, and when the droplets reached the droplet split the particles were directed into the centre daughter droplets. The results show that the design of the droplet split and the flow ratio between the centre and side outlet channels are the main factors that affect the particle enrichment and particle recovery in the centre daughter droplets. The highest particle enrichment was achieved in the droplet split design having the smallest centre channel (38 μm wide). Using this microfluidic chip design, we demonstrate up to 16.7-fold enrichment of 9.9 μm diameter polystyrene microbeads in the centre daughter droplets. This is almost three times higher particle enrichment than what has previously been presented using other intra-droplet particle enrichment techniques. Moreover, the acoustic technique is label-free and biocompatible.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Nanoteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Nano-technology (hsv//eng)
Nyckelord
- Acoustophoresis
- Acoustofluidics
- Bulk acoustic waves
- Droplet microfluidics
- Microfabrication
- Particle enrichment
- Teknisk fysik med inriktning mot mikrosystemteknik
- Engineering Science with specialization in Microsystems Technology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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