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Satellite DNA conte...
Satellite DNA content of B chromosomes in the characid fish Characidium gomesi supports their origin from sex chromosomes
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- Serrano-Freitas, Erica A. (författare)
- Univ Estadual Paulista, UNESP, Inst Biociencias Botucatu, Dept Morfol, BR-18618970 Botucatu, SP, Brazil;Fundacao Educ Penapolis, Ctr Ciencias Biol & Saude, BR-16303180 Penapolis, SP, Brazil
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- Silva, Duilio M. Z. A. (författare)
- Univ Estadual Paulista, UNESP, Inst Biociencias Botucatu, Dept Morfol, BR-18618970 Botucatu, SP, Brazil
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- Ruiz-Ruano, Francisco J. (författare)
- Uppsala universitet,Evolutionsbiologi,Univ Granada, Dept Genet, Granada 18071, Spain
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- Utsunomia, Ricardo (författare)
- Univ Fed Rural Rio de Janeiro, ICBS, Inst Ciencias Biol & Saude, Dept Genet, BR-23897000 Seropedica, RJ, Brazil
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- Araya-Jaime, Cristian (författare)
- Univ La Serena, Inst Invest Multidisciplinar Ciencia & Tecnol, La Serena 1720256, Chile;Univ La Serena, Dept Biol, Lab Genet & Citogenet Vegetal, La Serena 1720256, Chile
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- Oliveira, Claudio (författare)
- Univ Estadual Paulista, UNESP, Inst Biociencias Botucatu, Dept Morfol, BR-18618970 Botucatu, SP, Brazil
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- Camacho, Juan Pedro M. (författare)
- Univ Granada, Dept Genet, Granada 18071, Spain
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- Foresti, Fausto (författare)
- Univ Estadual Paulista, UNESP, Inst Biociencias Botucatu, Dept Morfol, BR-18618970 Botucatu, SP, Brazil
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(creator_code:org_t)
- 2019-10-17
- 2020
- Engelska.
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Ingår i: Molecular Genetics and Genomics. - : SPRINGER HEIDELBERG. - 1617-4615 .- 1617-4623. ; 295:1, s. 195-207
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The origin of supernumerary (B) chromosomes is clearly conditioned by their ancestry from the standard (A) chromosomes. Sequence similarity between A and B chromosomes is thus crucial to determine B chromosome origin. For this purpose, we compare here the DNA sequences from A and B chromosomes in the characid fish Characidium gomesi using two main approaches. First, we found 59 satellite DNA (satDNA) families constituting the satellitome of this species and performed FISH analysis for 18 of them. This showed the presence of six satDNAs on the B chromosome: one shared with sex chromosomes and autosomes, two shared with sex chromosomes, one shared with autosomes and two being B-specific. This indicated that B chromosomes most likely arose from the sex chromosomes. Our second approach consisted of the analysis of five repetitive DNA families: 18S and 5S ribosomal DNA (rDNA), the H3 histone gene, U2 snDNA and the most abundant satDNA (CgoSat01-184) on DNA obtained from microdissected B chromosomes and from B-lacking genomes. PCR and sequence analysis of these repetitive sequences was successful for three of them (5S rDNA, H3 histone gene and CgoSat01-184), and sequence comparison revealed that DNA sequences obtained from the B chromosomes displayed higher identity with C. gomesi genomic DNA than with those obtained from other Characidium species. Taken together, our results support the intraspecific origin of B chromosomes in C. gomesi and point to sex chromosomes as B chromosome ancestors, which raises interesting prospects for future joint research on the genetic content of sex and B chromosomes in this species.
Ämnesord
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
Nyckelord
- Next-generation sequencing
- Satellitome
- RepeatExplorer
- Fluorescence in situ hybridization
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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