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Heterogeneity of Hu...
Heterogeneity of Human Pancreatic Islet Isolation Around Europe : Results of a Survey Study
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- Nano, Rita (author)
- IRCCS, Diabet Res Inst, San Raffaele Sci Inst, Via Olgettina 60, I-20132 Milan, Italy.
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- Kerr-Conte, Julie A. (author)
- Univ Hosp Lille, European Genom Inst Diabet, Lille, France.
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- Scholz, Hanne (author)
- Oslo Univ Hosp, Dept Transplant Med, Oslo, Norway.
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- Engelse, Marten (author)
- Leiden Univ, Dept Nephrol, Med Ctr, Leiden, Netherlands.
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- Karlsson, Marie (author)
- Uppsala universitet,Klinisk immunologi
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- Saudek, Frantisek (author)
- Inst Clin & Expt Med, Diabet Ctr, Prague, Czech Republic.
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- Bosco, Domenico (author)
- Hop Univ Geneve, Geneva, Switzerland.
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- Antonioli, Barbara (author)
- ASST Grande Osped Metropolitano Niguarda, Diabetol Unit, Milan, Italy.
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- Bertuzzi, Federico (author)
- ASST Grande Osped Metropolitano Niguarda, Diabetol Unit, Milan, Italy.
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- Johnson, Paul R., V (author)
- Univ Oxford, Nuffield Dept Surg Sci, Islet Transplant Res Grp, Oxford, England.;Univ Oxford, Oxford Ctr Diabet, Oxford, England.
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- Ludwing, Barbara (author)
- Univ Hosp Carl Gustav Carus Dresden, Dept Med 3, Dresden, Germany.
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- Ling, Zhidong (author)
- Vrije Univ Brussel, Acad Hosp, Brussels, Belgium.;Vrije Univ Brussel, Diabet Res Ctr, Brussels, Belgium.
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- De Paep, Diedert L. (author)
- Vrije Univ Brussel, Acad Hosp, Brussels, Belgium.;Vrije Univ Brussel, Diabet Res Ctr, Brussels, Belgium.
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- Keymeulen, Bart (author)
- Vrije Univ Brussel, Acad Hosp, Brussels, Belgium.;Vrije Univ Brussel, Diabet Res Ctr, Brussels, Belgium.
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- Pattou, Francois (author)
- Univ Hosp Lille, European Genom Inst Diabet, Lille, France.
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- Berney, Thierry (author)
- Hop Univ Geneve, Geneva, Switzerland.
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- Korsgren, Olle (author)
- Uppsala universitet,Klinisk immunologi
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- de Koning, Eelco (author)
- Leiden Univ, Dept Nephrol, Med Ctr, Leiden, Netherlands.
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- Piemonti, Lorenzo (author)
- IRCCS, Diabet Res Inst, San Raffaele Sci Inst, Via Olgettina 60, I-20132 Milan, Italy.;Univ Vita Salute San Raffaele, Milan, Italy.
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IRCCS, Diabet Res Inst, San Raffaele Sci Inst, Via Olgettina 60, I-20132 Milan, Italy Univ Hosp Lille, European Genom Inst Diabet, Lille, France. (creator_code:org_t)
- LIPPINCOTT WILLIAMS & WILKINS, 2020
- 2020
- English.
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In: Transplantation. - : LIPPINCOTT WILLIAMS & WILKINS. - 0041-1337 .- 1534-6080. ; 104:1, s. 190-196
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Background: Europe is currently the most active region in the field of pancreatic islet transplantation, and many of the leading groups are actually achieving similar good outcomes. Further collaborative advances in the field require the standardization of islet cell product isolation processes, and this work aimed to identify differences in the human pancreatic islet isolation processes within European countries.Methods: A web-based questionnaire about critical steps, including donor selection, pancreas processing, pancreas perfusion and digestion, islet counting and culture, islet quality evaluation, microbiological evaluation, and release criteria of the product, was completed by isolation facilities participating at the Ninth International European Pancreas and Islet Transplant Association (EPITA) Workshop on Islet-Beta Cell Replacement in Milan.Results: Eleven islet isolation facilities completed the questionnaire. The facilities reported 445 and 53 islet isolations per year over the last 3 years from deceased organ donors and pancreatectomized patients, respectively. This activity resulted in 120 and 40 infusions per year in allograft and autograft recipients, respectively. Differences among facilities emerged in donor selection (age, cold ischemia time, intensive care unit length, amylase concentration), pancreas procurement, isolation procedures (brand and concentration of collagenase, additive, maximum acceptable digestion time), quality evaluation, and release criteria for transplantation (glucose-stimulated insulin secretion tests, islet numbers, and purity). Moreover, even when a high concordance about the relevance of one parameter was evident, thresholds for the acceptance were different among facilities.Conclusions: The result highlighted the presence of a heterogeneity in the islet cell product process and product release criteria.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Nano, Rita
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Kerr-Conte, Juli ...
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Scholz, Hanne
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Engelse, Marten
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Karlsson, Marie
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Saudek, Frantise ...
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show more...
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Bosco, Domenico
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Antonioli, Barba ...
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Bertuzzi, Federi ...
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Johnson, Paul R. ...
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Ludwing, Barbara
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Ling, Zhidong
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De Paep, Diedert ...
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Keymeulen, Bart
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Pattou, Francois
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Berney, Thierry
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Korsgren, Olle
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de Koning, Eelco
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Piemonti, Lorenz ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Endocrinology an ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Surgery
- Articles in the publication
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Transplantation
- By the university
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Uppsala University