Caesalpinia cristacoat extract protects red blood cell from sodium nitrite-induced oxidative stress and exhibits antiplatelet activity

Srinivasa, ChandrammaTumkur Univ, Dept Studies & Res Biochem, BH Rd, Tumakuru 572103, India. (författare)

To understand the RBC protecting efficiency and antiplatelet activity of methanolic extract ofCaesalpinia cristacoat (MECCC). RBC-protecting activity of MECCC was evaluated using assays, such as DPPH, level of lipid peroxidation, protein carbonyl content, superoxide dismutase and catalase as a marker of oxidative stress whereas, platelet aggregation inhibition was performed using human platelet-rich plasma (PRP). MECCC showed about 76% of DPPH-scavenging activity, with an IC(50)value of 71.89 mu g/ml. The MECCC reduced the level of lipid peroxidation and protein carboxylation in RBC caused by NaNO(2)in a dose-dependent manner. In addition, MECCC normalized the levels of superoxide dismutase (SOD) and catalase (CAT) in oxidative stress-induced RBC in a dose-dependent manner. This suggested the protective effect of MECCC on RBC against oxidative stress. Furthermore, MECCC also exhibited mild antiplatelet activity by inhibiting both ADP and epinephrine agonists that induced platelet aggregation. The noticed inhibition percentage was found to be 28 and 23%, respectively at the concentration of 150 mu g. Interestingly, MECCC did not hydrolyse the RBC suggesting its nontoxic properties. MECCC possesses protective effect of RBC against NaNO2(10 mmol/l) induce oxidative stress and inhibits platelet aggregation.

Kengaiah, JayannaTumkur Univ, Dept Studies & Res Biochem, BH Rd, Tumakuru 572103, India. (författare)
Nandish, Sharath Kumar M.Tumkur Univ, Dept Studies & Res Biochem, BH Rd, Tumakuru 572103, India. (författare)
Ramachandraiah, ChethanaTumkur Univ, Dept Studies & Res Biochem, BH Rd, Tumakuru 572103, India. (författare)
Hanumegowda, Sujatha M.Kuvempu University, Dept Biochem Jnansahydri, Shivamogga, Karnataka, India. (författare)
Shivaiah, AshwiniTumkur Univ, Dept Studies & Res Biochem, BH Rd, Tumakuru 572103, India. (författare)
Santhosh, SebestianUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi (författare)
Sannaningaiah, DevarajaTumkur Univ, Dept Studies & Res Biochem, BH Rd, Tumakuru 572103, India. (författare)
Tumkur Univ, Dept Studies & Res Biochem, BH Rd, Tumakuru 572103, India.Kuvempu University, Dept Biochem Jnansahydri, Shivamogga, Karnataka, India. (creator_code:org_t)

Ingår i:Blood Coagulation and Fibrinolysis: LIPPINCOTT WILLIAMS & WILKINS31:5, s. 293-3020957-52351473-5733

Av författaren/redakt...: Srinivasa, Chand ...; Kengaiah, Jayann ...; Nandish, Sharath ...; Ramachandraiah, ...; Hanumegowda, Suj ...; Shivaiah, Ashwin ...; visa fler...; Santhosh, Sebest ...; Sannaningaiah, D ...; visa färre...

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