Sökning: L773:1523 1747 OR L773:0022 202X
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Proliferating kerat...
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Széll, Márta
(författare)
Proliferating keratinocytes are putative sources of the psoriasis susceptibility-related EDA+ (extra domain A of fibronectin) oncofetal fibronectin.
- Artikel/kapitelEngelska2004
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Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-421717
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-421717URI
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https://doi.org/10.1111/j.0022-202X.2004.23224.xDOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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The extra domain A of fibronectin (EDA+ oncofetal isoform of fibronectin was recently reported to be overexpressed in psoriatic uninvolved epidermis. It has been proposed that the abnormal presence of EDA+ oncofetal protein at the dermal-epidermal junction in the uninvolved skin may provide the "psoriatic" environment in which keratinocytes are in a preactivated state with regard to mitogenic signals (e.g., T cell lymphokines). To determine the possible sources of cellular fibronectin in the non-lesional psoriatic skin, we aimed to investigate whether keratinocytes could produce the EDA+ oncofetal form of fibronectin. RT-PCR studies revealed that both cultured normal keratinocytes and HaCaT cells express the EDA+ splice variant of fibronectin mRNA, and in HaCaT cells the EDA+/EDA- transcript ratio was elevated compared with normal keratinocytes. Cultured keratinocytes and HaCaT cells showed intracytoplasmic staining with an EDA+ fibronectin-specific antibody and among the positively stained cells many showed mitosis. Using RT-PCR, western blot analysis, and flow cytometry, we showed that in synchronized HaCaT cells the amount of both total fibronectin and its EDA+ isoform change with the proliferation/differentiation state of HaCaT cells and peak in highly proliferating cells. We show that in short-term ex vivo cultures, a small population of EDA+ fibronectin containing cell population appear among psoriatic uninvolved, but not normal epidermal cells. We also demonstrate that cell attachment has a strong influence on the expression of both total and EDA+ fibronectin. Our results suggest that proliferating keratinocytes could be the sources of the psoriasis susceptibility-related EDA+ oncofetal fibronectin in the epidermis.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Bata-Csörgo, Zsuzsanna
(författare)
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Koreck, Andrea
(författare)
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Pivarcsi, Andor
(författare)
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Polyánka, Hilda
(författare)
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Szeg, Csilla
(författare)
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Gaál, Magdolna
(författare)
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Dobozy, Attila
(författare)
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Kemény, Lajos
(författare)
Sammanhörande titlar
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Ingår i:Journal of Investigative Dermatology123:3, s. 537-460022-202X1523-1747
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