Search: WFRF:(Sundberg Olle)
> (2020-2024) >
Multiplexing DNA me...
Multiplexing DNA methylation markers to detect circulating cell-free DNA derived from human pancreatic β cells
-
- Neiman, Daniel (author)
- Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, Hadassah Med Sch, Jerusalem, Israel.
-
- Gillis, David (author)
- Hadassah Hebrew Univ, Endocrine Unit, Dept Pediat, Med Ctr, Jerusalem, Israel.
-
- Piyanzin, Sheina (author)
- Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, Hadassah Med Sch, Jerusalem, Israel.
-
show more...
-
- Cohen, Daniel (author)
- Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, Hadassah Med Sch, Jerusalem, Israel.
-
- Fridlich, Ori (author)
- Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, Hadassah Med Sch, Jerusalem, Israel.
-
- Moss, Joshua (author)
- Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, Hadassah Med Sch, Jerusalem, Israel.
-
- Zick, Aviad (author)
- Hadassah Hebrew Univ, Sharett Inst Oncol, Med Ctr, Jerusalem, Israel.
-
- Oron, Tal (author)
- Schneider Childrens Med Ctr Israel, Inst Endocrinol & Diabet, Petah Tiqwa, Israel.
-
- Sundberg, Frida (author)
- Sahlgrens Univ Hosp, Queen Silvia Childrens Hosp, Gothenburg, Sweden.
-
- Forsander, Gun (author)
- Sahlgrens Univ Hosp, Queen Silvia Childrens Hosp, Gothenburg, Sweden.
-
- Skog, Oskar, Docent, PhD, 1981- (author)
- Uppsala universitet,Klinisk immunologi
-
- Korsgren, Olle (author)
- Uppsala universitet,Klinisk immunologi
-
- Levy-Khademi, Floris (author)
- Shaare Zedek Med Ctr, Dept Pediat, Jerusalem, Israel.
-
- Arbel, Dan (author)
- Hadassah Hebrew Univ, Med Ctr, Pediat Surg, Jerusalem, Israel.
-
- Hashavia, Saar (author)
- Hadassah Hebrew Univ, Med Ctr, Pediat Emergency, Jerusalem, Israel.
-
- Shapiro, A. M. James (author)
- Univ Alberta, Li Ka Shing Ctr Hlth Res Innovat, Edmonton, AB, Canada.
-
- Speake, Cate (author)
- Benaroya Res Inst, Diabet Program, Seattle, WA USA.
-
- Greenbaum, Carla (author)
- Benaroya Res Inst, Diabet Program, Seattle, WA USA.
-
- Hosford, Jennifer (author)
- Univ Florida, Diabet Inst, Dept Pediat, Gainesville, FL 32610 USA.;Univ Florida, Diabet Inst, Dept Pathol Immunol & Lab Med, Gainesville, FL 32610 USA.
-
- Posgai, Amanda (author)
- Univ Florida, Coll Med, Gainesville, FL 32610 USA.
-
- Atkinson, Mark A. (author)
- Univ Florida, Diabet Inst, Dept Pediat, Gainesville, FL 32610 USA.;Univ Florida, Diabet Inst, Dept Pathol Immunol & Lab Med, Gainesville, FL 32610 USA.;Univ Florida, Coll Med, Gainesville, FL 32610 USA.
-
- Glaser, Benjamin (author)
- Hadassah Hebrew Univ, Dept Internal Med, Endocrinol & Metab Serv, Med Ctr, Jerusalem, Israel.
-
- Schatz, Desmond A. (author)
- Univ Florida, Diabet Inst, Dept Pediat, Gainesville, FL 32610 USA.;Univ Florida, Diabet Inst, Dept Pathol Immunol & Lab Med, Gainesville, FL 32610 USA.
-
- Shemer, Ruth (author)
- Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, Hadassah Med Sch, Jerusalem, Israel.
-
- Dor, Yuval (author)
- Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, Hadassah Med Sch, Jerusalem, Israel.
-
show less...
-
Hebrew Univ Jerusalem, Inst Med Res Israel Canada, Dept Dev Biol & Canc Res, Hadassah Med Sch, Jerusalem, Israel Hadassah Hebrew Univ, Endocrine Unit, Dept Pediat, Med Ctr, Jerusalem, Israel. (creator_code:org_t)
- 2020-07-23
- 2020
- English.
-
In: JCI Insight. - : American Society for Clinical Investigation. - 2379-3708. ; 5:14
- Related links:
-
http://insight.jci.o...
-
show more...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- It has been proposed that unmethylated insulin promoter fragments in plasma derive exclusively from β cells, reflect their recent demise, and can be used to assess β cell damage in type 1 diabetes. Herein we describe an ultrasensitive assay for detection of a β cell–specific DNA methylation signature, by simultaneous assessment of 6 DNA methylation markers, that identifies β cell DNA in mixtures containing as little as 0.03% β cell DNA (less than 1 β cell genome equivalent). Based on this assay, plasma from nondiabetic individuals (N = 218, aged 4–78 years) contained on average only 1 β cell genome equivalent/mL. As expected, cell-free DNA (cfDNA) from β cells was significantly elevated in islet transplant recipients shortly after transplantation. We also detected β cell cfDNA in a patient with KATP congenital hyperinsulinism, in which substantial β cell turnover is thought to occur. Strikingly, in contrast to previous reports, we observed no elevation of β cell–derived cfDNA in autoantibody-positive subjects at risk for type 1 diabetes (N = 32), individuals with recent-onset type 1 diabetes (<4 months, N = 92), or those with long-standing disease (>4 months, N = 38). We discuss the utility of sensitive β cell cfDNA analysis and potential explanations for the lack of a β cell cfDNA signal in type 1 diabetes.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Neiman, Daniel
-
Gillis, David
-
Piyanzin, Sheina
-
Cohen, Daniel
-
Fridlich, Ori
-
Moss, Joshua
-
show more...
-
Zick, Aviad
-
Oron, Tal
-
Sundberg, Frida
-
Forsander, Gun
-
Skog, Oskar, Doc ...
-
Korsgren, Olle
-
Levy-Khademi, Fl ...
-
Arbel, Dan
-
Hashavia, Saar
-
Shapiro, A. M. J ...
-
Speake, Cate
-
Greenbaum, Carla
-
Hosford, Jennife ...
-
Posgai, Amanda
-
Atkinson, Mark A ...
-
Glaser, Benjamin
-
Schatz, Desmond ...
-
Shemer, Ruth
-
Dor, Yuval
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Endocrinology an ...
- Articles in the publication
-
JCI Insight
- By the university
-
Uppsala University