WFRF:(Wesley E)
Search: WFRF:(Wesley E) > Tuning the Baird ar...
- 26 of 39
- Previous record
- Next record
- To hitlist
Tuning the Baird aromatic triplet-state energy of cyclooctatetraene to maximize the self-healing mechanism in organic fluorophores
-
- Pati, Avik K. (author)
- Weill Cornell Med, Dept Physiol & Biophys, New York, NY 10065 USA.;St Jude Childrens Res Hosp, Dept Struct Biol, Memphis, TN 38105 USA.
-
- El Bakouri, Ouissam (author)
- Uppsala universitet,Syntetisk molekylär kemi
-
- Jockusch, Steffen (author)
- Columbia Univ, Dept Chem, New York, NY 10027 USA.
- show more...
- show less...
- Weill Cornell Med, Dept Physiol & Biophys, New York, NY 10065 USA;St Jude Childrens Res Hosp, Dept Struct Biol, Memphis, TN 38105 USA. Syntetisk molekylär kemi (creator_code:org_t)
- 2020-09-10
- 2020
- English.
- In: Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences (PNAS). - 0027-8424 .- 1091-6490. ; 117:39, s. 24305-24315
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Bright, photostable, and nontoxic fluorescent contrast agents are critical for biological imaging. "Self-healing" dyes, in which triplet states are intramolecularly quenched, enable fluorescence imaging by increasing fluorophore brightness and longevity, while simultaneously reducing the generation of reactive oxygen species that promote phototoxicity. Here, we systematically examine the self-healing mechanism in cyanine-class organic fluorophores spanning the visible spectrum. We show that the Baird aromatic triplet-state energy of cyclooctatetraene can be physically altered to achieve order of magnitude enhancements in fluorophore brightness and signal-to-noise ratio in both the presence and absence of oxygen. We leverage these advances to achieve direct measurements of large-scale conformational dynamics within single molecules at submillisecond resolution using wide-field illumination and camera-based detection methods. These findings demonstrate the capacity to image functionally relevant conformational processes in biological systems in the kilohertz regime at physiological oxygen concentrations and shed important light on the multivariate parameters critical to self-healing organic fluorophore design.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- self-healing fluorophore
- photostability
- Baird aromaticity
- cyclooctatetraene
- single-molecule imaging
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Proceedings of the National Academy of Sciences of the United States of America (Search for host publication in LIBRIS)
To the university's database
- 26 of 39
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Pati, Avik K.
- El Bakouri, Ouis ...
- Jockusch, Steffe ...
- Zhou, Zhou
- Altman, Roger B.
- Fitzgerald, Gabr ...
- show more...
- Asher, Wesley B.
- Terry, Daniel S.
- Borgia, Alessand ...
- Holsey, Michael ...
- Batchelder, Jake ...
- Abeywickrama, Ch ...
- Huddle, Brandt
- Rufa, Dominic
- Javitch, Jonatha ...
- Ottosson, Henrik
- Blanchard, Scott ...
- show less...
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Chemical Science ...
- and Analytical Chemi ...
- Articles in the publication
- Proceedings of t ...
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
