Development and characterization of 15 novel polymorphic microsatellite loci for two important bot flies (Diptera, Oestridae) by next-generation sequencing

Aleix-Mata, GaelJaen Univ, Dept Expt Biol, E-23071 Jaen, Spain.;Jaen Univ, Dept Anim & Plant Biol & Ecol, E-23071 Jaen, Spain. (författare)

Cephenemyia stimulatorandOestrus ovisare two important parasitic bot flies (Oestridae) species causing myiasis, with a potential negative impact on the welfare of the host. Using next-generation sequencing approach and bioinformatics tools, a large panel of possible microsatellites loci was obtained in both species. Primer pairs were designed for 15 selected microsatellite loci inC. stimulatorand other 15 loci inO. ovisfor PCR amplification. Loci amplification and analysis were performed in four populations of each species. The results demonstrated that all selected loci were polymorphic, with the number of alleles ranging from 2 to 6 per locus inC. stimulatorand 3 to 13 per locus inO. ovis. This is the first time to describe these microsatellite loci forC. stimulatorandO. ovis. These two sets of microsatellite markers could be further used for biogeographic and population genetics studies.

Exposito, MiriamJaen Univ, Dept Expt Biol, E-23071 Jaen, Spain. (författare)
Ruiz-Ruano, Francisco J.Uppsala universitet,Evolutionsbiologi,Granada Univ, Fac Sci, Dept Genet, E-18071 Granada, Spain.(Swepub:uu)fraru543 (författare)
Maria Lopez-Beceiro, AnaUniv Santiago de Compostela, Dept Anat Anim Prod & Vet Clin Sci, E-27002 Lugo, Spain. (författare)
Fidalgo, Luis E.Univ Santiago de Compostela, Dept Anat Anim Prod & Vet Clin Sci, E-27002 Lugo, Spain. (författare)
Martinez-Carrasco, CarlosMurcia Univ, Dept Anim Hlth, E-30100 Murcia, Spain. (författare)
Ruiz de Ybanez, Maria RocioMurcia Univ, Dept Anim Hlth, E-30100 Murcia, Spain. (författare)
Boos, MathieuNaturaconst, Res Agcy Appl Ecol, F-67270 Wilshausen, France.;Federat Natl Chasseurs, Pole Sci, 13 Rue Gen Leclerc, F-92136 Issy Les Moulineaux, France. (författare)
Perez, Jesus M.Jaen Univ, Dept Anim & Plant Biol & Ecol, E-23071 Jaen, Spain.;Wildlife Ecol & Hlth Grp WE&H, Barcelona, Spain. (författare)
Sanchez, AntonioJaen Univ, Dept Expt Biol, E-23071 Jaen, Spain. (författare)
Jaen Univ, Dept Expt Biol, E-23071 Jaen, Spain.;Jaen Univ, Dept Anim & Plant Biol & Ecol, E-23071 Jaen, Spain.Jaen Univ, Dept Expt Biol, E-23071 Jaen, Spain. (creator_code:org_t)

Ingår i:Parasitology Research: Springer Nature119:9, s. 2829-28350932-01131432-1955

Av författaren/redakt...: Aleix-Mata, Gael; Exposito, Miriam; Ruiz-Ruano, Fran ...; Maria Lopez-Bece ...; Fidalgo, Luis E.; Martinez-Carrasc ...; visa fler...; Ruiz de Ybanez, ...; Boos, Mathieu; Perez, Jesus M.; Sanchez, Antonio; visa färre...

Om ämnet

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.