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Effects of Lactobac...
Effects of Lactobacillus johnsonii and Lactobacillus reuteri on gut barrier function and heat shock proteins in intestinal porcine epithelial cells
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- Li, Hao, 1984- (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper,Swedish Univ Agr Sci, Dept Anim Nutr & Management, POB 7024, S-75007 Uppsala, Sweden.
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- Roos, Stefan (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology,Swedish Univ Agr Sci, Uppsala BioCtr, Dept Microbiol, Uppsala, Sweden.
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- Jonsson, Hans (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology,Swedish Univ Agr Sci, Uppsala BioCtr, Dept Microbiol, Uppsala, Sweden.
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- Ahl, David (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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- Dicksved, Johan (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för husdjurens utfodring och vård (HUV),Department of Animal Nutrition and Management,Swedish Univ Agr Sci, Dept Anim Nutr & Management, POB 7024, S-75007 Uppsala, Sweden.
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- Lindberg, Jan Erik (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för husdjurens utfodring och vård (HUV),Department of Animal Nutrition and Management,Swedish Univ Agr Sci, Dept Anim Nutr & Management, POB 7024, S-75007 Uppsala, Sweden.
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- Lundh, Torbjörn (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för husdjurens utfodring och vård (HUV),Department of Animal Nutrition and Management,Swedish Univ Agr Sci, Dept Anim Nutr & Management, POB 7024, S-75007 Uppsala, Sweden.
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- Liu, Haoyu (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för husdjurens utfodring och vård (HUV),Department of Animal Nutrition and Management,Uppsala University
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(creator_code:org_t)
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- 2015-04-06
- 2015
- Engelska.
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Ingår i: Physiological Reports. - : WILEY. - 2051-817X. ; 3:4
- Relaterad länk:
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https://doi.org/10.1...
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https://pub.epsilon.... (primary) (Raw object) (free)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://res.slu.se/i...
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Abstract
Ämnesord
Stäng
- Heat shock proteins (HSPs) are a set of highly conserved proteins that can serve as intestinal gate keepers in gut homeostasis. Here, effects of a probiotic, Lactobacillus rhamnosus GG (LGG), and two novel porcine isolates, Lactobacillus johnsonii strain P47-HY and Lactobacillus reuteri strain P43-HUV, on cyto-protective HSP expression and gut barrier function, were investigated in a porcine IPEC-J2 intestinal epithelial cell line model. The IPEC-J2 cells polarized on a permeable filter exhibited villus-like cell phenotype with development of apical microvilli. Western blot analysis detected HSP expression in IPEC-J2 and revealed that L. johnsonii and L. reuteri strains were able to significantly induce HSP27, despite high basal expression in IPEC-J2, whereas LGG did not. For HSP72, only the supernatant of L. reuteri induced the expression, which was comparable to the heat shock treatment, which indicated that HSP72 expression was more stimulus specific. The protective effect of lactobacilli was further studied in IPEC-J2 under an enterotoxigenic Escherichia coli (ETEC) challenge. ETEC caused intestinal barrier destruction, as reflected by loss of cell-cell contact, reduced IPEC-J2 cell viability and transepithelial electrical resistance, and disruption of tight junction protein zonula occludens-1. In contrast, the L. reuteri treatment substantially counteracted these detrimental effects and preserved the barrier function. L. johnsonii and LGG also achieved barrier protection, partly by directly inhibiting ETEC attachment. Together, the results indicate that specific strains of Lactobacillus can enhance gut barrier function through cytoprotective HSP induction and fortify the cell protection against ETEC challenge through tight junction protein modulation and direct interaction with pathogens.
Ämnesord
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Jordbruksvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Agricultural Science (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- Cytoprotective heat shock protein 27 and 72
- intestinal barrier integrity
- IPEC-J2 cell line
- probiotic lactobacilli
- tight junction protein
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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