Phosphoinositides control insulin secretion through multiple routes

• Glucose-stimulated insulin secretion from pancreatic beta cells is the sequence of events that starts with glucose uptake and ends with the fusion of insulin granules with the plasma membrane through Ca2+-triggered exocytosis. Phosphoinositides are minor components of all cellular membranes, yet play fundamental roles as regulators of many cellular processes. PI(4,5)P2 is the most abundant phosphoinositide in the plasma membrane, where it controls the activity of ion channels, endo- and exocytosis and cytoskeletal rearrangements. However, its role in the regulation of insulin secretion is unclear and there are support for both direct stimulatory and inhibitory effects. Using an optogenetic approach to acutely recruit a PI(4,5)P2 phosphatase to deplete the plasma membrane of PI(4,5)P2 in living beta cells, we found that this lipid was required to support voltage-dependent Ca2+-influx and glucose-stimulated insulin secretion. Consistently, depolarization-induced Ca2+-influx was instead augmented when the plasma membrane PI(4,5)P2 concentration was increased by light-dependent recruitment of a PI(4,5)P2-synthesizing enzyme. PI(4)P is another phosphoinositide residing in the plasma membrane and other intracellular membranes. In addition to serving as a precursor for PI(4,5)P2, PI(4)P is used to fuel lipid exchange reactions at membrane contacts sites, such as the PI(4)P/cholesterol exchange at the ER-Golgi interface catalyzed by OSBP. Sac2 is a PI(4)P phosphatase that is highly expressed in neuronal tissues and the pancreas, where it localizes to endosomes and participates in endosome maturation. We found that Sac2 additionally binds to insulin granules through interactions with granule PI(4)P and Rab3. Loss of Sac2 resulted in accumulation of both PI(4)P and cholesterol on the granule surface, impaired insulin granule docking to the plasma membrane and reduced insulin secretion. The cholesterol levels on insulin granules were normalized in cells with reduced OSBP expression, indicating that Sac2 and OSBP cooperate at insulin granules. Acute inhibition of OSBP by OSW-1 resulted in the redistribution of OSBP, and its ER localized receptor VAP-A, from the ER-Golgi interface to insulin granules. Similar to loss of Sac2, both siRNA-mediated knockdown and pharmacological inhibition of OSBP resulted in decreased insulin secretion. Together, these results show that Sac2, by negatively regulating granule PI(4)P, limits OSBP-mediated cholesterol transfer to insulin granules at ER–insulin granule contact sites. Type-2 diabetes is associated with impaired insulin granule docking and exocytosis as well as altered cholesterol homeostasis. We found that Sac2 expression was reduced in patients with type-2 diabetes, which may help to explain some of the hallmarks of this disease at the level of the beta cell and also form the basis for future interventions.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Annan medicin och hälsovetenskap (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Other Medical and Health Sciences (hsv//eng)

Nyckelord

Phosphoinositides

PI(4)P

PI(4

5)P2

Ca2

optogenetics

VDCC

Sac2

OSBP

OSW-1

cholesterol

membrane contact sites

Publikations- och innehållstyp

vet (ämneskategori)

dok (ämneskategori)