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  • Lehmann, Laura C.Uppsala universitet,Molekylär systembiologi,Science for Life Laboratory, SciLifeLab (author)

Mechanistic Insights into Regulation of the ALC1 Remodeler by the Nucleosome Acidic Patch

  • Article/chapterEnglish2020

Publisher, publication year, extent ...

  • Elsevier BV,2020
  • electronicrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-429896
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-429896URI
  • https://doi.org/10.1016/j.celrep.2020.108529DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

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  • De tre första författarna delar förstaförfattarskapet
  • Upon DNA damage, the ALC1/CHD1L nucleosome remodeling enzyme (remodeler) is activated by binding to poly(ADP-ribose). How activated ALC1 recognizes the nucleosome, as well as how this recognition is coupled to remodeling, is unknown. Here, we show that remodeling by ALC1 requires a wild-type acidic patch on the entry side of the nucleosome. The cryo-electron microscopy structure of a nucleosome-ALC1 linker complex reveals a regulatory linker segment that binds to the acidic patch. Mutations within this interface alter the dynamics of ALC1 recruitment to DNA damage and impede the ATPase and remodeling activities of ALC1. Full activation requires acidic patch-linker segment interactions that tether the remodeler to the nucleosome and couple ATP hydrolysis to nucleosome mobilization. Upon DNA damage, such a requirement may be used to modulate ALC1 activity via changes in the nucleosome acidic patches.

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Added entries (persons, corporate bodies, meetings, titles ...)

  • Bacic, LukaUppsala universitet,Molekylär systembiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)lukba790 (author)
  • Hewitt, Graeme (author)
  • Brackmann, KlausUppsala universitet,Science for Life Laboratory, SciLifeLab,Molekylär systembiologi(Swepub:uu)klabr476 (author)
  • Sabantsev, AntonUppsala universitet,Molekylär systembiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)antsa780 (author)
  • Gaullier, GuillaumeUppsala universitet,Molekylär systembiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)guiga936 (author)
  • Pytharopoulou, SofiaUppsala universitet,Science for Life Laboratory, SciLifeLab,Molekylär systembiologi(Swepub:uu)sofpy130 (author)
  • Degliesposti, Gianluca (author)
  • Okkenhaug, Hanneke (author)
  • Tan, Song (author)
  • Costa, Alessandro (author)
  • Skehel, J. Mark (author)
  • Boulton, Simon J. (author)
  • Deindl, SebastianUppsala universitet,Molekylär systembiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)sebde386 (author)
  • Uppsala universitetMolekylär systembiologi (creator_code:org_t)

Related titles

  • In:Cell Reports: Elsevier BV33:122211-1247

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