WFRF:(Stiasny K)
Sökning: WFRF:(Stiasny K) > (2020-2021) > High-throughput seq...
- 1 av 2
- Föregående post
- Nästa post
- Till träfflistan
High-throughput sequencing of two European strains of tick-borne encephalitis virus (TBEV), Hochosterwitz and 1993/783
-
- Paulsen, Katrine M. (författare)
- Norwegian Inst Publ Hlth, Div Infect Control & Environm Hlth, Dept Virol, POB 222 Skoyen, NO-0213 Oslo, Norway.
-
- Lamsal, Alaka (författare)
- Norwegian Inst Publ Hlth, Div Infect Control & Environm Hlth, Dept Virol, POB 222 Skoyen, NO-0213 Oslo, Norway.;Univ South Eastern Norway, Dept Nat Sci & Environm Hlth, Gullbringvegen 36, NO-3800 Bo, Norway.
-
- Bastakoti, Srijana (författare)
- Norwegian Inst Publ Hlth, Div Infect Control & Environm Hlth, Dept Virol, POB 222 Skoyen, NO-0213 Oslo, Norway.
- visa fler...
- visa färre...
- Norwegian Inst Publ Hlth, Div Infect Control & Environm Hlth, Dept Virol, POB 222 Skoyen, NO-0213 Oslo, Norway Norwegian Inst Publ Hlth, Div Infect Control & Environm Hlth, Dept Virol, POB 222 Skoyen, NO-0213 Oslo, Norway.;Univ South Eastern Norway, Dept Nat Sci & Environm Hlth, Gullbringvegen 36, NO-3800 Bo, Norway. (creator_code:org_t)
- Elsevier, 2021
- 2021
- Engelska.
- Ingår i: Ticks and Tick-borne Diseases. - : Elsevier. - 1877-959X .- 1877-9603. ; 12:1
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- Tick-borne encephalitis virus (TBEV) is a medically important arbovirus, widespread in Europe and Asia. The virus is primarily transmitted to humans and animals by bites from ticks and, in rare cases, by consumption of unpasteurized dairy products. The aim of this study was to sequence and characterize two TBEV strains with amplicon sequencing by designing overlapping primers. The amplicon sequencing, via Illumina MiSeq, covering nearly the entire TBEV genome, was successful: We retrieved and characterized the complete polyprotein sequence of two TBEV strains, Hochosterwitz and 1993/783 from Austria and Sweden, respectively. In this study the previous phylogenetic analysis of both strains was confirmed to be of the European subtypes of TBEV (TBEV-Eu) by whole genome sequencing. The Hochosterwitz strain clustered with the two strains KrM 93 and KrM 213 from South Korea, and the 1993/783 strain clustered together with the NL/UH strain from the Netherlands. Our study confirms the suitability and rapidness of the high-throughput sequencing method used to produce complete TBEV genomes from TBEV samples of high viral load giving high-molecular-weight cDNA with large overlapping amplicons.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Nyckelord
- TBEV
- Flaviviruses
- WGS
- Phylogeny
- Ambiguous nucleotide
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
- Ticks and Tick-borne Diseases (Sök värdpublikationen i LIBRIS)
Till lärosätets databas
- 1 av 2
- Föregående post
- Nästa post
- Till träfflistan
Hitta mer i SwePub
- Av författaren/redakt...
- Paulsen, Katrine ...
- Lamsal, Alaka
- Bastakoti, Srija ...
- Pettersson, John ...
- Pedersen, Benedi ...
- Stiasny, Karin
- visa fler...
- Haglund, Mats
- Smura, Teemu
- Vapalahti, Olli
- Vikse, Rose
- Alfsnes, Kristia ...
- Andreassen, Ashi ...
- visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
- MEDICIN OCH HÄLS ...
- och Klinisk medicin
- och Infektionsmedici ...
- Artiklar i publikationen
- Ticks and Tick-b ...
- Av lärosätet
- Uppsala universitet
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
