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  • Alim, Abdul,1983-Uppsala universitet,Institutionen för folkhälso- och vårdvetenskap,Institutionen för medicinsk biokemi och mikrobiologi,Uppsala Univ, Sweden (author)

Glutamate triggers the expression of functional ionotropic and metabotropic glutamate receptors in mast cells

  • Article/chapterEnglish2021

Publisher, publication year, extent ...

  • 2020-04-20
  • Springer Nature,2021
  • electronicrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-434116
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-434116URI
  • https://doi.org/10.1038/s41423-020-0421-zDOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-165570URI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:143505012URI
  • https://res.slu.se/id/publ/105417URI

Supplementary language notes

  • Language:English
  • Summary in:English

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Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Funding Agencies|AFA Forsakring; Swedish Research CouncilSwedish Research Council; Swedish Cancer Foundation; Swedish Heart and Lung FoundationSwedish Heart-Lung Foundation; Swedish Child Cancer Foundation; Knut and Alice Wallenberg FoundationKnut & Alice Wallenberg Foundation; regional agreement on medical training and clinical research (ALF)
  • Mast cells are emerging as players in the communication between peripheral nerve endings and cells of the immune system. However, it is not clear the mechanism by which mast cells communicate with peripheral nerves. We previously found that mast cells located within healing tendons can express glutamate receptors, raising the possibility that mast cells may be sensitive to glutamate signaling. To evaluate this hypothesis, we stimulated primary mast cells with glutamate and showed that glutamate induced the profound upregulation of a panel of glutamate receptors of both the ionotropic type (NMDAR1, NMDAR2A, and NMDAR2B) and the metabotropic type (mGluR2 and mGluR7) at both the mRNA and protein levels. The binding of glutamate to glutamate receptors on the mast cell surface was confirmed. Further, glutamate had extensive effects on gene expression in the mast cells, including the upregulation of pro-inflammatory components such as IL-6 and CCL2. Glutamate also induced the upregulation of transcription factors, including Egr2, Egr3 and, in particular, FosB. The extensive induction of FosB was confirmed by immunofluorescence assessment. Glutamate receptor antagonists abrogated the responses of the mast cells to glutamate, supporting the supposition of a functional glutamate-glutamate receptor axis in mast cells. Finally, we provide in vivo evidence supporting a functional glutamate-glutamate receptor axis in the mast cells of injured tendons. Together, these findings establish glutamate as an effector of mast cell function, thereby introducing a novel principle for how cells in the immune system can communicate with nerve cells.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Grujic, MirjanaUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Uppsala Univ, Sweden(Swepub:uu)mirgr704 (author)
  • Ackerman, Paul WDepartment of Molecular Medicine and Surgery, Karolinska Institute, Stockholm, Sweden,Karolinska Inst, Sweden (author)
  • Kristiansson, PerUppsala universitet,Allmänmedicin och preventivmedicin,Uppsala Univ, Sweden(Swepub:uu)perkrist (author)
  • Eliasson, Pernilla T.Linköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten(Swepub:liu)perel67 (author)
  • Peterson, Magnus,1966-Uppsala universitet,Allmänmedicin och preventivmedicin,Uppsala Univ, Sweden; Acad Primary Hlth Care, Sweden(Swepub:uu)mapet227 (author)
  • Pejler, GunnarSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Uppsala Univ, Sweden; Swedish Univ Agr Sci, Sweden,Institutionen för anatomi, fysiologi och biokemi,Department of Anatomy, Physiology and Biochemistry (AFB),Uppsala University(Swepub:slu)49530 (author)
  • Uppsala universitetInstitutionen för folkhälso- och vårdvetenskap (creator_code:org_t)
  • Sveriges lantbruksuniversitet

Related titles

  • In:Cellular & Molecular Immunology: Springer Nature18:10, s. 2383-23921672-76812042-0226

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