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Multidrug Antimicro...
Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken
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- Uddin, Md Bashir (författare)
- Sylhet Agr Univ, Dept Med, Sylhet 3100, Bangladesh.
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- Hossain, S. M. Bayejed (författare)
- Sylhet Agr Univ, Dept Med, Sylhet 3100, Bangladesh.
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- Hasan, Mahmudul (författare)
- Sylhet Agr Univ, Dept Pharmaceut & Ind Biotechnol, Sylhet 3100, Bangladesh.
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- Alam, Mohammad Nurul (författare)
- Sylhet Agr Univ, Dept Med, Sylhet 3100, Bangladesh.
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- Debnath, Mita (författare)
- Kazi Farms Poultry Lab, Gazipur 1700, Bangladesh.
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- Begum, Ruhena (författare)
- Bangladesh Livestock Res Inst, Dhaka 1341, Bangladesh.
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- Roy, Sawrab (författare)
- Sylhet Agr Univ, Dept Microbiol & Immunol, Sylhet 3100, Bangladesh.
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- Harun-Al-Rashid, Ahmed (författare)
- Sylhet Agr Univ, Dept Aquat Resource Management, Sylhet 3100, Bangladesh.
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- Chowdhury, Md. Shahidur Rahman (författare)
- Sylhet Agr Univ, Dept Med, Sylhet 3100, Bangladesh.
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- Rahman, Md. Mahfujur (författare)
- Sylhet Agr Univ, Dept Med, Sylhet 3100, Bangladesh.
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- Hossain, Md. Mukter (författare)
- Sylhet Agr Univ, Dept Med, Sylhet 3100, Bangladesh.
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- Elahi, Fazle (författare)
- Kangwon Natl Univ, Dept Food Sci & Biotechnol, Chunchon 200701, South Korea.
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- Chowdhury, Mohammed Yousuf Elahi (författare)
- Chattogram Vet & Anim Sci Univ, Dept Med & Surg, Chattogram 4225, Bangladesh.
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- Järhult, Josef D., 1975- (författare)
- Uppsala universitet,Infektionsmedicin,Zoonosis Science Center
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- El Zowalaty, Mohamed E. (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Univ Sharjah, Dept Clin Sci, Coll Med, Sharjah 27272, U Arab Emirates.,Zoonosis Science Center
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- Ahmed, Syed Sayeem Uddin (författare)
- Sylhet Agr Univ, Dept Epidemiol & Publ Hlth, Sylhet 3100, Bangladesh.
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Sylhet Agr Univ, Dept Med, Sylhet 3100, Bangladesh Sylhet Agr Univ, Dept Pharmaceut & Ind Biotechnol, Sylhet 3100, Bangladesh. (creator_code:org_t)
- 2021-01-15
- 2021
- Engelska.
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Ingår i: Animals. - : MDPI. - 2076-2615. ; 11:1
- Relaterad länk:
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://www.mdpi.com...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Colistin (polymyxin E) is widely used in animal and human medicine and is increasingly used as one of the last-resort antibiotics against Gram-negative bacilli. Due to the increased use of colistin in treating infections caused by multidrug-resistant Gram-negative bacteria, resistance to this antibiotic ought to be monitored. The study was undertaken to elucidate the molecular mechanisms, genetic relationships and phenotype correlations of colistin-resistant isolates. Here, we report the detection of the mcr-1 gene in chicken-associated Salmonella isolates in Bangladesh and its in-silico functional analysis. Out of 100 samples, 82 Salmonella spp. were isolated from chicken specimens (liver, intestine). Phenotypic disc diffusion and minimum inhibitory concentration (MIC) assay using different antimicrobial agents were performed. Salmonella isolates were characterized using PCR methods targeting genus-specific invA and mcr-1 genes with validation for the functional analysis. The majority of the tested Salmonella isolates were found resistant to colistin (92.68%), ciprofloxacin (73.17%), tigecycline (62.20%) and trimethoprim/sulfamethoxazole (60.98%). When screened using PCR, five out of ten Salmonella isolates were found to carry the mcr-1 gene. One isolate was confirmed for Salmonella enterica subsp. enterica serovar Enteritidis, and other four isolates were confirmed for Salmonella enterica subsp. enterica serovar Typhimurium. Sequencing and phylogenetic analysis revealed a divergent evolutionary relationship between the catalytic domain of Neisseria meningitidis lipooligosaccharide phosphoethanolamine transferase A (LptA) and MCR proteins, rendering them resistant to colistin. Three-dimensional homology structural analysis of MCR-1 proteins and molecular docking interactions suggested that MCR-1 and LptA share a similar substrate binding cavity, which could be validated for the functional analysis. The comprehensive molecular and in-silico analyses of the colistin resistance mcr-1 gene of Salmonella spp. of chicken origin in the present study highlight the importance of continued monitoring and surveillance for antimicrobial resistance among pathogens in food chain animals.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- antimicrobial resistance
- Enterobacteriaceae
- colistin
- mcr-1 gene
- Salmonella enterica
- foodborne
- poultry
- Bangladesh
- chicken
- phosphoethanolamine
- LptA
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Uddin, Md Bashir
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Hossain, S. M. B ...
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Hasan, Mahmudul
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Alam, Mohammad N ...
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Debnath, Mita
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Begum, Ruhena
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Roy, Sawrab
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Harun-Al-Rashid, ...
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Chowdhury, Md. S ...
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Rahman, Md. Mahf ...
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Hossain, Md. Muk ...
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Elahi, Fazle
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Chowdhury, Moham ...
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Järhult, Josef D ...
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El Zowalaty, Moh ...
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Ahmed, Syed Saye ...
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Uppsala universitet