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  • Rocha, Elerson MatosUniv Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil. (författare)

Culturable bacteria associated with Anopheles darlingi and their paratransgenesis potential

  • Artikel/kapitelEngelska2021

Förlag, utgivningsår, omfång ...

  • 2021-01-13
  • BioMed Central (BMC),2021
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:uu-435198
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-435198URI
  • https://doi.org/10.1186/s12936-020-03574-1DOI
  • https://res.slu.se/id/publ/109965URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Correction in: Malaria Journal, vol. 20, issue 1, article number 83.DOI: 10.1186/s12936-021-03609-1
  • Background: Malaria remains a major public health problem in South America, mostly in the Amazon region. Among newly proposed ways of controlling malaria transmission to humans, paratransgenesis is a promising alternative. Paratransgenesis aims to inhibit the development of parasites within the vector through the action of genetically modified bacteria. The first step towards successful paratransgenesis in the Amazon is the identification of Anopheles darlingi symbiotic bacteria, which are transmitted vertically among mosquitoes, and are not pathogenic to humans.Methods: Culturable bacteria associated with An. darlingi and their breeding sites were isolated by conventional microbiological techniques. Isolated strains were transformed with a GFP expressing plasmid, pSPT-1-GFP, and reintroduced in mosquitoes by feeding. Their survival and persistence in the next generation was assessed by the isolation of fluorescent bacteria from eggs, larvae, pupae and adult homogenates.Results: A total of 179 bacterial strains were isolated from samples from two locations, Coari and Manaus. The predominant genera identified in this study were Acinetobacter, Enterobacter, Klebsiella, Serratia, Bacillus, Elizabethkingia, Stenotrophomonas and Pantoea. Two isolated strains, Serratia-Adu40 and Pantoea-Ovo3, were successfully transformed with the pSPT-1-GFP plasmid and expressed GFP. The fluorescent bacteria fed to adult females were transferred to their eggs, which persisted in larvae and throughout metamorphosis, and were detected in adult mosquitoes of the next generation.Conclusion: Serratia-Adu40 and Pantoea-Ovo3 are promising candidates for paratransgenesis in An. darlingi. Further research is needed to determine if these bacteria are vertically transferred in nature.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Marinotti, OsvaldoMTEKPrime, Aliso Viejo, CA USA. (författare)
  • Serrao, Deidre MachadoUniv Estadual Amazonas MBT, UEA, Manaus, Amazonas, Brazil. Univ Estadual Amazonas BIONORTE, UEA, Manaus, Amazonas, Brazil. (författare)
  • Correa, Laura VianaUniv Estadual Amazonas MBT, UEA, Manaus, Amazonas, Brazil. Univ Estadual Amazonas BIONORTE, UEA, Manaus, Amazonas, Brazil. (författare)
  • Katak, Ricardo de MeloUniv Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil. (författare)
  • Juan, Campos-de-OliveirUniv Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil. (författare)
  • Muniz, Veranilce AlvesUniv Estadual Amazonas MBT, UEA, Manaus, Amazonas, Brazil. Univ Estadual Amazonas BIONORTE, UEA, Manaus, Amazonas, Brazil. (författare)
  • de Oliveira, Marta Rodrigues (författare)
  • Ferreira, JoaquimINPA, Inst Nacl Pesquisas Amazonia, Lab Malaria & Dengue, Manaus, Amazonas, Brazil. (författare)
  • Pessoa, Marcos Cezar FernandesUniv Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil. (författare)
  • Roque, Rosemary AparecidaINPA, Inst Nacl Pesquisas Amazonia, Lab Malaria & Dengue, Manaus, Amazonas, Brazil. (författare)
  • da Mota, Adolfo JoseUniv Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil. (författare)
  • Onorati, PieroSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för ekologi,Department of Ecology,Swedish Univ Agr Sci SLU, Dept Ecol, Box 7044, S-75007 Uppsala, Sweden. (författare)
  • Souza-Neto, Jayme A.Sao Paulo State Univ UNESP, Cent Multi User Lab, Dept Bioproc & Biotechnol, Sch Agr Sci, Botucatu, SP, Brazil. (författare)
  • Terenius, OlleSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Mikrobiologi,Swedish Univ Agr Sci SLU, Dept Ecol, Box 7044, S-75007 Uppsala, Sweden.,Institutionen för ekologi,Department of Ecology,Uppsala University(Swepub:slu)47992 (författare)
  • Tadei, Wanderli PedroINPA, Inst Nacl Pesquisas Amazonia, Lab Malaria & Dengue, Manaus, Amazonas, Brazil. (författare)
  • Univ Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil.MTEKPrime, Aliso Viejo, CA USA. (creator_code:org_t)
  • Sveriges lantbruksuniversitet

Sammanhörande titlar

  • Ingår i:Malaria Journal: BioMed Central (BMC)201475-2875

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