Search: (WFRF:(Alanentalo Tomas)) conttype:(refereed) >
Mesoscopic 3D imagi...
Mesoscopic 3D imaging of pancreatic cancer and Langerhans islets based on tissue autofluorescence
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- Hahn, Max (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM),Umeå Univ, Umeå Ctr Mol Med, Johan Bures Vag 12, S-90187 Umeå, Sweden.
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- Nord, Christoffer (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM),Umeå Univ, Umeå Ctr Mol Med, Johan Bures Vag 12, S-90187 Umeå, Sweden.
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- Franklin, Oskar, 1985- (author)
- Umeå universitet,Kirurgi,Umeå Univ, Dept Med Biosci, Umeå, Sweden.
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- Alanentalo, Tomas (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM),Umeå Univ, Umeå Ctr Mol Med, Johan Bures Vag 12, S-90187 Umeå, Sweden.
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- Eriksson, Maria (author)
- Umeå universitet,Patologi,Umeå centrum för molekylär medicin (UCMM),Umeå Univ, Dept Med Biosci, Umeå, Sweden.,Umeå Univ, Umeå Ctr Mol Med, Johan Bures Vag 12, S-90187 Umeå, Sweden.
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- Morini, Federico (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM),Umeå Univ, Umeå Ctr Mol Med, Johan Bures Vag 12, S-90187 Umeå, Sweden.
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- Eriksson, Maria (author)
- Umeå Univ, Umeå Ctr Mol Med, Johan Bures Vag 12, S-90187 Umeå, Sweden.
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- Korsgren, Olle (author)
- Uppsala universitet,Klinisk immunologi
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- Sund, Malin (author)
- Umeå universitet,Kirurgi,Department of Surgical and Perioperative Sciences/Surgery, Umeå University Hospital, Umeå, Swede,Umeå Univ, Dept Med Biosci, Umeå, Sweden.;Umeå Univ Hosp, Dept Surg & Perioperat Sci Surg, Umeå, Sweden.
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- Ahlgren, Ulf (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM),Umeå Univ, Umeå Ctr Mol Med, Johan Bures Vag 12, S-90187 Umeå, Sweden.
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(creator_code:org_t)
- 2020-10-26
- 2020
- English.
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In: Scientific Reports. - : NATURE RESEARCH. - 2045-2322. ; 10
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Abstract
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- The possibility to assess pancreatic anatomy with microscopic resolution in three dimensions (3D) would significantly add to pathological analyses of disease processes. Pancreatic ductal adenocarcinoma (PDAC) has a bleak prognosis with over 90% of the patients dying within 5 years after diagnosis. Cure can be achieved by surgical resection, but the efficiency remains drearily low. Here we demonstrate a method that without prior immunohistochemical labelling provides insight into the 3D microenvironment and spread of PDAC and premalignant cysts in intact surgical biopsies. The method is based solely on the autofluorescent properties of the investigated tissues using optical projection tomography and/or light-sheet fluorescence microscopy. It does not interfere with subsequent histopathological analysis and may facilitate identification of tumor-free resection margins within hours. We further demonstrate how the developed approach can be used to assess individual volumes and numbers of the islets of Langerhans in unprecedently large biopsies of human pancreatic tissue, thus providing a new means by which remaining islet mass may be assessed in settings of diabetes. Generally, the method may provide a fast approach to provide new anatomical insight into pancreatic pathophysiology.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Gastroenterologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
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- ref (subject category)
- art (subject category)
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- By the author/editor
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Hahn, Max
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Nord, Christoffe ...
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Franklin, Oskar, ...
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Alanentalo, Toma ...
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Eriksson, Maria
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Morini, Federico
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Korsgren, Olle
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Sund, Malin
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Ahlgren, Ulf
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Surgery
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Gastroenterology ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
- Articles in the publication
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Scientific Repor ...
- By the university
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Uppsala University
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Umeå University