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Electrolyte handlin...
Electrolyte handling in the isolated perfused rat kidney : demonstration of vasopressin V2-receptor-dependent calcium reabsorption
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- Bamberg, Krister (författare)
- AstraZeneca, BioPharmaceut R&D, Translat Sci & Expt Med, Res & Early Dev,Cardiovasc Renal & Metab, Gothenburg, Sweden.
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- William-Olsson, Lena (författare)
- AstraZeneca, BioPharmaceut R&D, Biosci Renal Res & Early Dev, Cardiovasc Renal & Metab, Gothenburg, Sweden.
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- Johansson, Ulrika (författare)
- AstraZeneca, BioPharmaceut R&D, Biosci Renal Res & Early Dev, Cardiovasc Renal & Metab, Gothenburg, Sweden.
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- Arner, Anders (författare)
- Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Hartleib-Geschwindner, Judith (författare)
- AstraZeneca, BioPharmaceut R&D, Projects Res & Early Dev, Cardiovasc Renal & Metab, Gothenburg, Sweden.
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- Sällström, Johan (författare)
- Uppsala University,Karolinska Institute,Uppsala universitet,Institutionen för medicinsk cellbiologi,Karolinska Inst, Dept Physiol & Pharmacol, Stockholm, Sweden.
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AstraZeneca, BioPharmaceut R&D, Translat Sci & Expt Med, Res & Early Dev,Cardiovasc Renal & Metab, Gothenburg, Sweden AstraZeneca, BioPharmaceut R&D, Biosci Renal Res & Early Dev, Cardiovasc Renal & Metab, Gothenburg, Sweden. (creator_code:org_t)
- 2020-08-19
- 2020
- Engelska.
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Ingår i: Upsala Journal of Medical Sciences. - : TAYLOR & FRANCIS LTD. - 0300-9734 .- 2000-1967. ; 125:4, s. 274-280
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Abstract
Ämnesord
Stäng
- Background The most profound effect of vasopressin on the kidney is to increase water reabsorption through V-2-receptor (V2R) stimulation, but there are also data suggesting effects on calcium transport. To address this issue, we have established an isolated perfused kidney model with accurate pressure control, to directly study the effects of V2R stimulation on kidney function, isolated from systemic effects. Methods The role of V2R in renal calcium handling was studied in isolated rat kidneys using a new pressure control system that uses a calibration curve to compensate for the internal pressure drop up to the tip of the perfusion cannula. Results Kidneys subjected to V2R stimulation using desmopressin (DDAVP) displayed stable osmolality and calcium reabsorption throughout the experiment, whereas kidneys not administered DDAVP exhibited a simultaneous fall in urine osmolality and calcium reabsorption. Epithelial sodium channel (ENaC) inhibition using amiloride resulted in a marked increase in potassium reabsorption along with decreased sodium reabsorption. Conclusions A stable isolated perfused kidney model with computer-controlled pressure regulation was developed, which retained key physiological functions. The preparation responds to pharmacological inhibition of ENaC channels and activation of V2R. Using the model, the dynamic effects of V2R stimulation on calcium handling and urine osmolality could be visualised. The study thereby provides evidence for a stimulatory role of V2R in renal calcium reabsorption.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Urologi och njurmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Urology and Nephrology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Nyckelord
- AVP
- ENaC
- kidney
- vasopressin
- AVP
- ENaC
- kidney
- vasopressin
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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