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A microfluidics-bas...
A microfluidics-based method for culturing osteoblasts on biomimetic hydroxyapatite
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- Atif, Abdul Raouf, 1996- (författare)
- Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,EMBLA
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- Pujari-Palmer, Michael (författare)
- Uppsala universitet,Tillämpad materialvetenskap
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- Tenje, Maria (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Mikrosystemteknik,EMBLA
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- Mestres, Gemma, 1984- (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Mikrosystemteknik,EMBLA
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(creator_code:org_t)
- Elsevier, 2021
- 2021
- Engelska.
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Ingår i: Acta Biomaterialia. - : Elsevier. - 1742-7061 .- 1878-7568. ; 127, s. 327-337
- Relaterad länk:
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https://urn.kb.se/re...
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Abstract
Ämnesord
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- The reliability of conventional cell culture studies to evaluate biomaterials is often questioned, as in vitro outcomes may contradict results obtained through in vivo assays. Microfluidics technology has the potential to reproduce complex physiological conditions by allowing for fine control of microscale features such as cell confinement and flow rate. Having a continuous flow during cell culture is especially advantageous for bioactive biomaterials such as calcium-deficient hydroxyapatite (HA), which may otherwise alter medium composition and jeopardize cell viability, potentially producing false negative results in vitro. In this work, HA was integrated into a microfluidics-based platform (HA-on-chip) and the effect of varied flow rates (2, 8 and 14 µl/min, corresponding to 0.002, 0.008 and 0.014 dyn/cm2, respectively) was evaluated. A HA sample placed in a well plate (HA-static) was included as a control. While substantial calcium depletion and phosphate release occurred in static conditions, the concentration of ions in HA-on-chip samples remained similar to those of fresh medium, particularly at higher flow rates. Pre-osteoblast-like cells (MC3T3-E1) exhibited a significantly higher degree of proliferation on HA-on-chip (8 μl/min flow rate) as compared to HA-static. However, cell differentiation, analysed by alkaline phosphatase (ALP) activity, showed low values in both conditions. This study indicates that cells respond differently when cultured on HA under flow compared to static conditions, which indicates the need for more physiologically relevant methods to increase the predictive value of in vitro studies used to evaluate biomaterials.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Nyckelord
- Calcium phosphate cement
- Flow
- In vitro
- On-chip
- Shear stress
- Teknisk fysik med inriktning mot mikrosystemteknik
- Engineering Science with specialization in Microsystems Technology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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