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VEGF-B Promotes End...
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Räsänen, MarkusUniv Helsinki, Fac Med, Wihuri Res Inst, Helsinki, Finland.;Univ Helsinki, Fac Med, Translat Canc Med Program, Helsinki, Finland
(författare)
VEGF-B Promotes Endocardium-Derived Coronary Vessel Development and Cardiac Regeneration
- Artikel/kapitelEngelska2021
Förlag, utgivningsår, omfång ...
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Wolters Kluwer,2021
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-442274
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-442274URI
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https://doi.org/10.1161/CIRCULATIONAHA.120.050635DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:146294441URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Background:Recent discoveries have indicated that, in the developing heart, sinus venosus and endocardium provide major sources of endothelium for coronary vessel growth that supports the expanding myocardium. Here we set out to study the origin of the coronary vessels that develop in response to vascular endothelial growth factor B (VEGF-B) in the heart and the effect of VEGF-B on recovery from myocardial infarction.Methods:We used mice and rats expressing a VEGF-B transgene, VEGF-B-gene–deleted mice and rats, apelin-CreERT, and natriuretic peptide receptor 3–CreERT recombinase-mediated genetic cell lineage tracing and viral vector–mediated VEGF-B gene transfer in adult mice. Left anterior descending coronary vessel ligation was performed, and 5-ethynyl-2’-deoxyuridine–mediated proliferating cell cycle labeling; flow cytometry; histological, immunohistochemical, and biochemical methods; single-cell RNA sequencing and subsequent bioinformatic analysis; microcomputed tomography; and fluorescent- and tracer-mediated vascular perfusion imaging analyses were used to study the development and function of the VEGF-B–induced vessels in the heart.Results:We show that cardiomyocyte overexpression of VEGF-B in mice and rats during development promotes the growth of novel vessels that originate directly from the cardiac ventricles and maintain connection with the coronary vessels in subendocardial myocardium. In adult mice, endothelial proliferation induced by VEGF-B gene transfer was located predominantly in the subendocardial coronary vessels. Furthermore, VEGF-B gene transduction before or concomitantly with ligation of the left anterior descending coronary artery promoted endocardium-derived vessel development into the myocardium and improved cardiac tissue remodeling and cardiac function.Conclusions:The myocardial VEGF-B transgene promotes the formation of endocardium-derived coronary vessels during development, endothelial proliferation in subendocardial myocardium in adult mice, and structural and functional rescue of cardiac tissue after myocardial infarction. VEGF-B could provide a new therapeutic strategy for cardiac neovascularization after coronary occlusion to rescue the most vulnerable myocardial tissue.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Sultan, IbrahimUniv Helsinki, Fac Med, Wihuri Res Inst, Helsinki, Finland.;Univ Helsinki, Fac Med, Translat Canc Med Program, Helsinki, Finland
(författare)
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Paech, JenniferUniv Helsinki, Fac Med, Wihuri Res Inst, Helsinki, Finland.;Univ Helsinki, Fac Med, Translat Canc Med Program, Helsinki, Finland
(författare)
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Hemanthakumar, Karthik AmudhalaUniv Helsinki, Fac Med, Wihuri Res Inst, Helsinki, Finland.;Univ Helsinki, Fac Med, Translat Canc Med Program, Helsinki, Finland
(författare)
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Yu, WeiUniv Chinese Acad Sci, Chinese Acad Sci, State Key Lab Cell Biol, Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem, Beijing, Peoples R China
(författare)
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He, LiqunUppsala universitet,Vaskulärbiologi,Tianjin Med Univ Gen Hosp, Dept Neurosurg, Tianjin Neurol Inst,Minist Educ & Tianjin City, Key Lab Postneuroinjury Neurorepair & Regenerat C, Tianjin, Peoples R China(Swepub:uu)liqhe592
(författare)
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Tang, JuanUniv Chinese Acad Sci, Chinese Acad Sci, State Key Lab Cell Biol, Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem, Beijing, Peoples R China
(författare)
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Sun, YingUppsala universitet,Vaskulärbiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)yisun086
(författare)
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Hlushchuk, RuslanUniv Bern, Inst Anat, Bern, Switzerland
(författare)
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Huan, XiuzhengUniv Chinese Acad Sci, Chinese Acad Sci, State Key Lab Cell Biol, Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem, Beijing, Peoples R China
(författare)
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Armstrong, EmmaUniv Helsinki, Fac Med, Wihuri Res Inst, Helsinki, Finland; Univ Helsinki, Fac Med, Translat Canc Med Program, Helsinki, Finland
(författare)
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Khoma, Oleksiy-ZakharUniv Bern, Inst Anat, Bern, Switzerland
(författare)
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Mervaala, EeroUniv Helsinki, Fac Med, Helsinki, Finland
(författare)
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Djonov, ValentinUniv Bern, Inst Anat, Bern, Switzerland
(författare)
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Betsholtz, ChristerUppsala universitet,Vaskulärbiologi,Karolinska Inst, Integrated Cardio Metab Ctr, Huddinge, Sweden(Swepub:uu)chbet517
(författare)
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Zhou, BinUniv Chinese Acad Sci, Chinese Acad Sci, State Key Lab Cell Biol, Ctr Excellence Mol Cell Sci,Shanghai Inst Biochem, Beijing, Peoples R China
(författare)
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Kivelä, RiikkaUniv Helsinki, Fac Med, Wihuri Res Inst, Helsinki, Finland.;Univ Helsinki, Fac Med, Translat Canc Med Program, Helsinki, Finland
(författare)
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Alitalo, KariUniv Helsinki, Fac Med, Wihuri Res Inst, Helsinki, Finland; Univ Helsinki, Fac Med, Translat Canc Med Program, Helsinki, Finland
(författare)
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Univ Helsinki, Fac Med, Wihuri Res Inst, Helsinki, Finland.;Univ Helsinki, Fac Med, Translat Canc Med Program, Helsinki, FinlandUniv Chinese Acad Sci, Chinese Acad Sci, State Key Lab Cell Biol, Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem, Beijing, Peoples R China
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Circulation: Wolters Kluwer143:1, s. 65-770009-73221524-4539
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Till lärosätets databas
- Av författaren/redakt...
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Räsänen, Markus
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Sultan, Ibrahim
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Paech, Jennifer
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Hemanthakumar, K ...
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Yu, Wei
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He, Liqun
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visa fler...
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Tang, Juan
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Sun, Ying
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Hlushchuk, Rusla ...
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Huan, Xiuzheng
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Armstrong, Emma
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Khoma, Oleksiy-Z ...
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Mervaala, Eero
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Djonov, Valentin
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Betsholtz, Chris ...
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Zhou, Bin
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Kivelä, Riikka
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Alitalo, Kari
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visa färre...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Kardiologi
- Artiklar i publikationen
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Circulation
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Uppsala universitet
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Karolinska Institutet