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Cubosomes for topic...
Cubosomes for topical delivery of the antimicrobial peptide LL-37
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- Boge, Lukas, 1987 (author)
- Chalmers University of Technology,Research Institutes of Sweden (RISE)
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- Hallstensson, Karin (author)
- RISE Research Institutes of Sweden,Research Institutes of Sweden (RISE)
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- Ringstad, Lovisa (author)
- RISE Research Institutes of Sweden,Research Institutes of Sweden (RISE)
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- Johansson, Jenny (author)
- RISE Research Institutes of Sweden,Research Institutes of Sweden (RISE)
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- Andersson, Therese (author)
- RISE Research Institutes of Sweden,Research Institutes of Sweden (RISE)
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- Davoudi, Mina (author)
- Lund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Larsson, Per Tomas (author)
- Innventia AB
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- Mahlapuu, Margit, 1972 (author)
- University of Gothenburg,Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine,Promore Pharma AB
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- Håkansson, Joakim (author)
- RISE Research Institutes of Sweden,Research Institutes of Sweden (RISE)
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- Andersson, Martin, 1974 (author)
- Chalmers University of Technology
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(creator_code:org_t)
- Elsevier BV, 2019
- 2019
- English.
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In: European Journal of Pharmaceutics and Biopharmaceutics. - : Elsevier BV. - 1873-3441 .- 0939-6411. ; 134, s. 60-67
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Abstract
Subject headings
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- In this study, the use of cubosomes for topical delivery of the antimicrobial peptide (AMP) LL-37 was investigated. Topical delivery of AMPs is of great interest for treatment of skin infections caused by bacteria, such as Staphylococcus aureus. AMP containing cubosomes were produced by three different preparation protocols and compared: (i) pre-loading, where LL-37 was incorporated into a liquid crystalline gel, which thereafter was dispersed into nanoparticles, (ii) post-loading, where LL-37 was let to adsorb onto pre-formed cubosomes, and (iii) hydrotrope-loading, where LL-37 was incorporated during the spontaneously formed cubosomes in an ethanol/glycerol monooleate mixture. Particle size and size distribution were analyzed using dynamic light scattering (DLS), liquid crystalline structure by small angle x-ray scattering (SAXS) and release of LL-37 by a fluorescamine assay. Proteolytic protection of LL-37 as well as bactericidal effect after enzyme exposure was investigated. The skin irritation potential of cubosomes was examined by an in vitro epidermis model. Finally, the bacterial killing property of the cubosomes was examined by an ex vivo pig skin wound infection model with Staphylococcus aureus. Data showed that a high loading of LL-37 induced formation of vesicles in case of cubosomes prepared by sonication (pre-loading). No release of LL-37 was observed from the cubosomes, indicating strong association of the peptide to the particles. Proteolysis studies showed that LL-37 was fully protected against enzymatic attacks while associated with the cubosomes, also denoting strong association of the peptide to the particles. As a consequence, bactericidal effect after enzyme exposure remained, compared to pure LL-37 which was subjected to proteolysis. No skin irritation potential of the cubosomes was found, thus enabling for topical administration. The ex vivo wound infection model showed that LL-37 in pre-loaded cubosomes killed bacteria most efficient.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmaceutiska vetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmaceutical Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
- NATURVETENSKAP -- Kemi -- Annan kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Other Chemistry Topics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)
Keyword
- Skin irritation
- Cubosome
- Proteolysis
- LL-37
- Infection
- Topical delivery
- Antimicrobial peptide
- Antimicrobial peptide
- Cubosome
- Infection
- LL-37
- Proteolysis
- Skin irritation
- Topical delivery
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Boge, Lukas, 198 ...
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Hallstensson, Ka ...
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Ringstad, Lovisa
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Johansson, Jenny
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Andersson, There ...
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Davoudi, Mina
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Larsson, Per Tom ...
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Mahlapuu, Margit ...
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Håkansson, Joaki ...
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Andersson, Marti ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Pharmaceutical S ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Physical Chemist ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Other Chemistry ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Dermatology and ...
- Articles in the publication
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European Journal ...
- By the university
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Chalmers University of Technology
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University of Gothenburg
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Lund University
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Karolinska Institutet