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Lipopolysaccharide-Induced Nitric Oxide and Prostaglandin E2 Production Is Inhibited by Tellimagrandin II in Mouse and Human Macrophages
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- Lin, Chun-Yu (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Anestesiologi och intensivvård,Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Internal Med, Div Infect Dis, Kaohsiung 807, Taiwan.;Kaohsiung Med Univ, Ctr Trop Med & Infect Dis Res, Grad Inst Med, Sch Med,Coll Med, Kaohsiung 807, Taiwan
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- Kao, Shih-Han (author)
- Kaohsiung Med Univ, Ctr Trop Med & Infect Dis Res, Grad Inst Med, Sch Med,Coll Med, Kaohsiung 807, Taiwan.
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- Hung, Ling-Chien (author)
- Kaohsiung Med Univ, Ctr Trop Med & Infect Dis Res, Grad Inst Med, Sch Med,Coll Med, Kaohsiung 807, Taiwan.
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- Chien, Hsin-Ju (author)
- Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Internal Med, Div Infect Dis, Kaohsiung 807, Taiwan.
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- Wang, Wen-Hung (author)
- Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Internal Med, Div Infect Dis, Kaohsiung 807, Taiwan.;Kaohsiung Med Univ, Ctr Trop Med & Infect Dis Res, Grad Inst Med, Sch Med,Coll Med, Kaohsiung 807, Taiwan.
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- Chang, Yu-Wei (author)
- Kaohsiung Med Univ, Ctr Trop Med & Infect Dis Res, Grad Inst Med, Sch Med,Coll Med, Kaohsiung 807, Taiwan.
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- Chen, Yen-Hsu (author)
- Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Internal Med, Div Infect Dis, Kaohsiung 807, Taiwan.;Kaohsiung Med Univ, Ctr Trop Med & Infect Dis Res, Grad Inst Med, Sch Med,Coll Med, Kaohsiung 807, Taiwan.;Natl Chiao Tung Univ, Coll Biol Sci & Technol, Dept Biol Sci & Technol, Hsinchu 300, Taiwan.
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(creator_code:org_t)
- 2021-04-30
- 2021
- English.
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In: Life. - : MDPI. - 2075-1729. ; 11:5
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https://doi.org/10.3...
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Abstract
Subject headings
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- Sepsis develops from a serious microbial infection that causes the immune system to go into overdrive. The major microorganisms that induce sepsis are Gram-negative bacteria with lipopolysaccharide (LPS) in their cell walls. Nitric oxide (NO) and cyclooxygenase-2 (COX-2) are the key factors involved in the LPS-induced pro-inflammatory process. This study aimed to evaluate the effects of polyphenol Tellimagrandin II (TGII) on anti-inflammatory activity and its underlying basic mechanism in murine macrophage cell line RAW 264.7 and human monocyte-derived macrophages. Macrophages with more than 90% cell viability were found in the cytotoxicity assay under 50 mu M TGII. Pre- or post-treatment with TGII significantly reduced LPS-induced inducible nitric oxide synthase (NOS2) protein and mRNA expression, reducing LPS-induced COX-2 protein. Downstream of NOS2 and COX-2, NO and prostaglandin E2 (PGE2) were significantly inhibited by TGII. Upstream of NOS2 and COX-2, phospho-p65, c-fos and phospho-c-jun were also reduced after pre-treatment with TGII. Mitogen-activated protein kinases (MAPKs) are also critical to nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B) stimulation, and phospho-p38 expression was found to have been blocked by TGII. TGII efficiently reduces LPS-induced NO production and its upstream regulatory factors, suggesting that TGII may be a potential therapeutic agent for sepsis and other inflammatory diseases.
Subject headings
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
Keyword
- Tellimagrandin II
- macrophage
- inflammation
- nitric oxide
- cyclooxygenase-2
Publication and Content Type
- ref (subject category)
- art (subject category)
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