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- Noiri, MakotoUniv Tokyo, Dept Bioengn, Sch Engn, Tokyo 1138656, Japan (författare)
Exogenous Cell Surface Modification with Cell Penetrating Peptide-Conjugated Lipids Causes Spontaneous Cell Adhesion
- Artikel/kapitelEngelska2021
Förlag, utgivningsår, omfång ...
- 2021-04-30
- American Chemical Society (ACS),2021
- printrdacarrier
Nummerbeteckningar
- LIBRIS-ID:oai:DiVA.org:uu-446902
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-446902URI
- https://doi.org/10.1021/acsabm.1c00335DOI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
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- SwePubSwePub
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Anmärkningar
- The technique of cell patterning on a substrate is of great importance for platforms in cell-based assays. Chemical treatment of the substrate is commonly performed for cell patterning using cationic polymers, extracellular matrices, and antibodies. However, cell patterning could be easier if there is an approach to immobilize cells without treating the substrate surface. We previously reported that cell adhesion could be induced by the modification of the cellular surface with a cell-penetrating peptide (CPP)-conjugated poly(ethylene glycol)-phospholipid (CPP-PEG-lipid). This approach does not require chemical modification of the substrate surface, such as polystyrene or glass, and can be used for the cell patterning of floating cells. Here, we aimed to study the mechanism of induced cell adhesion using a representative CPP, Tat peptide (Tat-PEG-lipid). We found that cell adhesion was induced via electrostatic interactions between the Tat peptide and the substrate surface, which could be induced more efficiently by increasing the molecular weight of PEG together with CPPs but not with cationic peptides. The excluded volume effect between neighboring PEG chains could stretch the cell shape better than PEG with lower molecular weight, allowing the cell to spread firmly. In addition, Tat-PEG-lipid did not activate actin filament formation and did not influence the expression of focal adhesion kinase. Thus, the induced cell adhesion by CPP-PEG-lipid did not affect internal cell signaling.
Ämnesord och genrebeteckningar
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- cell adhesion
- PEG-lipid
- cell surface modification
- cell-penetrating peptide
- Tat peptide
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Goto, YuyaShibaura Inst Technol, Dept Biosci & Engn, Coll Syst Engn & Sci, Saitama 3378570, Japan (författare)
- Sato, YuyaUniv Tokyo, Dept Bioengn, Sch Engn, Tokyo 1138656, Japan (författare)
- Nakamura, NaokoShibaura Inst Technol, Dept Biosci & Engn, Coll Syst Engn & Sci, Saitama 3378570, Japan (författare)
- Ishihara, KazuhikoUniv Tokyo, Sch Engn, Dept Mat Engn, Tokyo 1138656, Japan (författare)
- Teramura, YujiUppsala universitet,Klinisk immunologi,Univ Tokyo, Dept Bioengn, Sch Engn, Tokyo 1138656, Japan(Swepub:uu)yujte186 (författare)
- Univ Tokyo, Dept Bioengn, Sch Engn, Tokyo 1138656, JapanShibaura Inst Technol, Dept Biosci & Engn, Coll Syst Engn & Sci, Saitama 3378570, Japan (creator_code:org_t)
Sammanhörande titlar
- Ingår i:ACS Applied Bio Materials: American Chemical Society (ACS)4:5, s. 4598-46062576-6422
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- ACS Applied Bio Materials (Sök värdpublikationen i LIBRIS)
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