Stromal Heterogeneity in the Human Proliferative Endometrium-A Single-Cell RNA Sequencing Study

Queckboerner, SuzannaKarolinska Inst, Div Obstet & Gynecol, Dept Womens & Childrens Hlth, S-17164 Solna, Sweden.;Karolinska Univ Hosp, S-17164 Solna, Sweden. (författare)

The endometrium undergoes regular regeneration and stromal proliferation as part of the normal menstrual cycle. To better understand cellular interactions driving the mechanisms in endometrial regeneration we employed single-cell RNA sequencing. Endometrial biopsies were obtained during the proliferative phase of the menstrual cycle from healthy fertile women and processed to single-cell suspensions which were submitted for sequencing. In addition to known endometrial cell types, bioinformatic analysis revealed multiple stromal populations suggestive of specific stromal niches with the ability to control inflammation and extracellular matrix composition. Ten different stromal cells and two pericyte subsets were identified. Applying different R packages (Seurat, SingleR, Velocyto) we established cell cluster diversity and cell lineage/trajectory, while using external data to validate our findings. By understanding healthy regeneration in the described stromal compartments, we aim to identify points of further investigation and possible targets for novel therapy development for benign gynecological disorders affecting endometrial regeneration and proliferation such as endometriosis and Asherman's syndrome.

von Grothusen, CarolinaKarolinska Institutet (författare)
Boggavarapu, Nageswara RaoKarolinska Institutet (författare)
Francis, Roy M.Uppsala universitet,Evolutionsbiologi,Uppsala Univ, BMC, Dept Med Biochem & Microbiol IMBIM, S-75237 Uppsala, Sweden.;Uppsala Univ, Dept Cell & Mol Biol ICM, Natl Bioinformat Infrastruct Sweden NBIS, SciLifeLab, S-75124 Uppsala, Sweden.(Swepub:uu)royfr474 (författare)
Davies, Lindsay C.Karolinska Inst, Dept Lab Med, S-14152 Huddinge, Sweden. (författare)
Gemzell-Danielsson, KristinaKarolinska Institutet (författare)
Karolinska InstitutetKarolinska Inst, Div Obstet & Gynecol, Dept Womens & Childrens Hlth, S-17164 Solna, Sweden.;Karolinska Univ Hosp, S-17164 Solna, Sweden. (creator_code:org_t)

Av författaren/redakt...: Queckboerner, Su ...; von Grothusen, C ...; Boggavarapu, Nag ...; Francis, Roy M.; Davies, Lindsay ...; Gemzell-Danielss ...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Cell och molekyl ...

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