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Proteomic Profiling...
Proteomic Profiling of Tissue Exosomes Indicates Continuous Release of Malignant Exosomes in Urinary Bladder Cancer Patients, Even with Pathologically Undetectable Tumour
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- Eldh, Maria (författare)
- Karolinska Institutet,Division of Immunology and Allergy, Department of Medicine Solna, Karolinska Institute, Stockholm, Sweden
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- Mints, Michael (författare)
- Umeå universitet,Karolinska Institutet,Urologi och andrologi,Division of Immunology and Allergy, Department of Medicine Solna, Karolinska Institute, Stockholm, Sweden
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- Hiltbrunner, Stefanie (författare)
- Karolinska Inst, Dept Med Solna, Div Immunol & Allergy, S-17164 Stockholm, Sweden.,Division of Immunology and Allergy, Department of Medicine Solna, Karolinska Institute, Stockholm, Sweden
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- Ladjevardi, Sam (författare)
- Uppsala universitet,Urologkirurgi,Akademiska Univ Hosp, Dept Urol, S-75185 Uppsala, Sweden.,Department of Urology, Akademiska University Hospital, Uppsala, Sweden
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- Alamdari, Farhood (författare)
- Vastmanland Hosp, Dept Urol, S-72189 Västerås, Sweden.,Department of Urology, Västmanland Hospital, Västerås, Sweden
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- Johansson, Markus (författare)
- Umeå universitet,Urologi och andrologi
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- Jakubczyk, Tomasz (författare)
- Lanssjukhuset Ryhov, Dept Urol, S-55305 Jönköping, Sweden.,Department of Urology, Länssjukhuset Ryhov, Jönköping, Sweden
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- Veerman, Rosanne E. (författare)
- Karolinska Institutet,Division of Immunology and Allergy, Department of Medicine Solna, Karolinska Institute, Stockholm, Sweden
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- Winqvist, Ola (författare)
- Karolinska Univ Hosp, Dept Clin Immunol & Transfus Med, S-17176 Solna, Sweden.,Department of Clinical Immunology and Transfusion Medicine, Karolinska University Hospital, Solna, Sweden
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- Sherif, Amir (författare)
- Umeå universitet,Urologi och andrologi
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- Gabrielsson, Susanne (författare)
- Karolinska Institutet,Division of Immunology and Allergy, Department of Medicine Solna, Karolinska Institute, Stockholm, Sweden
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(creator_code:org_t)
- 2021-06-29
- 2021
- Engelska.
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Ingår i: Cancers. - : MDPI. - 2072-6694. ; 13:13
- Relaterad länk:
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://www.mdpi.com...
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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http://kipublication...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Simple Summary Urinary bladder cancer (UBC) has a high recurrence rate, and biomarkers for different treatment strategies are highly needed. This study investigated the release of nanovesicles called exosomes from urinary bladder tissue from tumour-proximal sites as well as tumour-distant sites in transurethrally resected (TUR-B) patients with or without preoperative neoadjuvant chemotherapy prior to ensuing radical cystectomy-all without remaining visible tumour after TUR-B. We show that cancer-promoting exosomes were detected from both sites, suggesting that the previous tumour has altered the whole bladder tissue into a cancer-supporting milieu. The exosomes may originate from remaining pathologically undetectable cancer cells or transformed epithelial cells, and the study supports the notion of exosomes as mediators of metastatic spread and as potential biomarkers. It also supports early and radical removal of the bladder in urinary bladder cancer patients. Invasive urothelial bladder cancer (UBC) has high recurrence rates even after radical cystectomy (RC). Exosomes are membrane-bound nanovesicles, which have been shown to contribute to carcinogenesis and metastasis. We previously showed that urinary exosomes display a malignant profile in UBC patients despite the absence of detectable tumour. Here, we investigated exosomes from sampling sites close to or distant from the former tumour, aiming to understand the effect of the tumour on the local milieu. Ten patients scheduled for cystectomy after transurethral bladder resection (TUR-B), without remaining detectable tumour, were included. Exosomes were isolated from tissue explants of both the previous tumour site and distant bladder tissue. Proteins were quantified by mass spectrometry in seven patients. Exosomes from the previous tumour site were enriched in inflammatory but not cancer-related pathways compared to distant tissue. However, the 69 most abundant proteins in tissue-derived exosomes regardless of site, 20 of which were also found in urinary exosomes from our previous study, were enriched for cancer-related metabolic pathways and associated with poor prognosis in an external mRNA dataset. The enrichment of cancer-related pathways in the most abundant proteins, regardless of sampling site, confirms our hypothesis that despite the absence of detectable tumour, the entire bladder releases exosomes that contribute to metastasis and highlights the need for early RC.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Urologi och njurmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Urology and Nephrology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- extracellular vesicles
- exosomes
- urinary bladder neoplasms
- neoadjuvant therapy
- cystectomy
- proteomics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
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Cancers
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Till lärosätets databas
- Av författaren/redakt...
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Eldh, Maria
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Mints, Michael
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Hiltbrunner, Ste ...
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Ladjevardi, Sam
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Alamdari, Farhoo ...
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Johansson, Marku ...
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Jakubczyk, Tomas ...
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Veerman, Rosanne ...
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Winqvist, Ola
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Sherif, Amir
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Gabrielsson, Sus ...
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Urologi och njur ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Cancer och onkol ...
- Artiklar i publikationen
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Cancers
- Av lärosätet
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Uppsala universitet
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Karolinska Institutet
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Umeå universitet