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X-ray free-electron...
X-ray free-electron laser studies reveal correlated motion during isopenicillin N synthase catalysis
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- Rabe, Patrick (författare)
- Univ Oxford, Dept Chem, Chem Res Lab, 12 Mansfield Rd, Oxford OX1 3TA, England.;Univ Oxford, Ineos Oxford Inst Antimicrobial Res, 12 Mansfield Rd, Oxford OX1 3TA, England.
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- Kamps, Jos J. A. G. (författare)
- Univ Oxford, Dept Chem, Chem Res Lab, 12 Mansfield Rd, Oxford OX1 3TA, England.;Univ Oxford, Ineos Oxford Inst Antimicrobial Res, 12 Mansfield Rd, Oxford OX1 3TA, England.;Diamond Light Source, Diamond House,Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England.;Res Complex Harwell, Rutherford Appleton Lab, Didcot OX11 0FA, Oxon, England.
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- Sutherlin, Kyle D. (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, 1 Cyclotron Rd, Berkeley, CA 94720 USA.
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visa fler...
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- Linyard, James D. S. (författare)
- Univ Oxford, Dept Chem, Chem Res Lab, 12 Mansfield Rd, Oxford OX1 3TA, England.;Univ Oxford, Ineos Oxford Inst Antimicrobial Res, 12 Mansfield Rd, Oxford OX1 3TA, England.
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- Aller, Pierre (författare)
- Res Complex Harwell, Rutherford Appleton Lab, Didcot OX11 0FA, Oxon, England.
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- Pham, Cindy C. (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, 1 Cyclotron Rd, Berkeley, CA 94720 USA.
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- Makita, Hiroki (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, 1 Cyclotron Rd, Berkeley, CA 94720 USA.
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- Clifton, Ian (författare)
- Univ Oxford, Dept Chem, Chem Res Lab, 12 Mansfield Rd, Oxford OX1 3TA, England.;Univ Oxford, Ineos Oxford Inst Antimicrobial Res, 12 Mansfield Rd, Oxford OX1 3TA, England.
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- McDonough, Michael A. (författare)
- Univ Oxford, Dept Chem, Chem Res Lab, 12 Mansfield Rd, Oxford OX1 3TA, England.;Univ Oxford, Ineos Oxford Inst Antimicrobial Res, 12 Mansfield Rd, Oxford OX1 3TA, England.
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- Leissing, Thomas M. (författare)
- Univ Oxford, Dept Chem, Chem Res Lab, 12 Mansfield Rd, Oxford OX1 3TA, England.;Univ Oxford, Ineos Oxford Inst Antimicrobial Res, 12 Mansfield Rd, Oxford OX1 3TA, England.
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- Shutin, Denis (författare)
- Univ Oxford, Dept Chem, Chem Res Lab, 12 Mansfield Rd, Oxford OX1 3TA, England.;Univ Oxford, Ineos Oxford Inst Antimicrobial Res, 12 Mansfield Rd, Oxford OX1 3TA, England.
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- Lang, Pauline A. (författare)
- Univ Oxford, Dept Chem, Chem Res Lab, 12 Mansfield Rd, Oxford OX1 3TA, England.;Univ Oxford, Ineos Oxford Inst Antimicrobial Res, 12 Mansfield Rd, Oxford OX1 3TA, England.
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- Butryn, Agata (författare)
- Diamond Light Source, Diamond House,Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England.;Res Complex Harwell, Rutherford Appleton Lab, Didcot OX11 0FA, Oxon, England.
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- Brem, Jurgen (författare)
- Univ Oxford, Dept Chem, Chem Res Lab, 12 Mansfield Rd, Oxford OX1 3TA, England.;Univ Oxford, Ineos Oxford Inst Antimicrobial Res, 12 Mansfield Rd, Oxford OX1 3TA, England.
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- Gul, Sheraz (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, 1 Cyclotron Rd, Berkeley, CA 94720 USA.
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- Fuller, Franklin D. (författare)
- SLAC Natl Accelerator Lab, Linac Coherent Light Source, Menlo Pk, CA 94025 USA.
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Kim, In-Sik (författare)
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- Cheah, Mun Hon (författare)
- Uppsala universitet,Molekylär biomimetik
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- Fransson, Thomas (författare)
- Heidelberg Univ, Interdisciplinary Ctr Sci Comp, D-69120 Heidelberg, Germany.
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- Bhowmick, Asmit (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, 1 Cyclotron Rd, Berkeley, CA 94720 USA.
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- Young, Iris D. (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, 1 Cyclotron Rd, Berkeley, CA 94720 USA.;Univ Calif San Francisco, Dept Bioengn & Therapeut Sci, 600 16th St, San Francisco, CA 94158 USA.
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- O'Riordan, Lee (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, 1 Cyclotron Rd, Berkeley, CA 94720 USA.
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- Brewster, Aaron S. (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, 1 Cyclotron Rd, Berkeley, CA 94720 USA.
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- Pettinati, Ilaria (författare)
- Univ Oxford, Dept Chem, Chem Res Lab, 12 Mansfield Rd, Oxford OX1 3TA, England.;Univ Oxford, Ineos Oxford Inst Antimicrobial Res, 12 Mansfield Rd, Oxford OX1 3TA, England.
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- Doyle, Margaret (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, 1 Cyclotron Rd, Berkeley, CA 94720 USA.
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- Joti, Yasumasa (författare)
- Japan Synchrotron Radiat Res Inst, 1-1-1 Kouto, Sayo, Hyogo 6795198, Japan.;RIKEN SPring 8 Ctr, I-1-1 Kouto, Sayo, Hyogo 6795148, Japan.
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- Owada, Shigeki (författare)
- Japan Synchrotron Radiat Res Inst, 1-1-1 Kouto, Sayo, Hyogo 6795198, Japan.;RIKEN SPring 8 Ctr, I-1-1 Kouto, Sayo, Hyogo 6795148, Japan.
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- Tono, Kensuke (författare)
- Japan Synchrotron Radiat Res Inst, 1-1-1 Kouto, Sayo, Hyogo 6795198, Japan.;RIKEN SPring 8 Ctr, I-1-1 Kouto, Sayo, Hyogo 6795148, Japan.
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Batyuk, Alexander (författare)
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Hunter, Mark S. (författare)
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- Alonso-Mori, Roberto (författare)
- SLAC Natl Accelerator Lab, Linac Coherent Light Source, Menlo Pk, CA 94025 USA.
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- Bergmann, Uwe (författare)
- Stanford PULSE Inst, SLAC Natl Accelerator Lab, Menlo Pk, CA 94025 USA.;Univ Wisconsin Madison, Dept Phys, 1150 Univ Ave, Madison, WI 53706 USA.
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- Owen, Robin L. (författare)
- Diamond Light Source, Diamond House,Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England.
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- Sauter, Nicholas K. (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, 1 Cyclotron Rd, Berkeley, CA 94720 USA.
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- Claridge, Timothy D. W. (författare)
- Univ Oxford, Dept Chem, Chem Res Lab, 12 Mansfield Rd, Oxford OX1 3TA, England.;Univ Oxford, Ineos Oxford Inst Antimicrobial Res, 12 Mansfield Rd, Oxford OX1 3TA, England.
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- Robinson, Carol V. (författare)
- Univ Oxford, Dept Chem, Chem Res Lab, 12 Mansfield Rd, Oxford OX1 3TA, England.;Univ Oxford, Ineos Oxford Inst Antimicrobial Res, 12 Mansfield Rd, Oxford OX1 3TA, England.
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- Yachandra, Vittal K. (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, 1 Cyclotron Rd, Berkeley, CA 94720 USA.
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- Yano, Junko (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, 1 Cyclotron Rd, Berkeley, CA 94720 USA.
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- Kern, Jan F. (författare)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, 1 Cyclotron Rd, Berkeley, CA 94720 USA.
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- Orville, Allen M. (författare)
- Diamond Light Source, Diamond House,Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England.;Res Complex Harwell, Rutherford Appleton Lab, Didcot OX11 0FA, Oxon, England.
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- Schofield, Christopher J. (författare)
- Univ Oxford, Dept Chem, Chem Res Lab, 12 Mansfield Rd, Oxford OX1 3TA, England.;Univ Oxford, Ineos Oxford Inst Antimicrobial Res, 12 Mansfield Rd, Oxford OX1 3TA, England.
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Univ Oxford, Dept Chem, Chem Res Lab, 12 Mansfield Rd, Oxford OX1 3TA, England;Univ Oxford, Ineos Oxford Inst Antimicrobial Res, 12 Mansfield Rd, Oxford OX1 3TA, England. Univ Oxford, Dept Chem, Chem Res Lab, 12 Mansfield Rd, Oxford OX1 3TA, England.;Univ Oxford, Ineos Oxford Inst Antimicrobial Res, 12 Mansfield Rd, Oxford OX1 3TA, England.;Diamond Light Source, Diamond House,Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England.;Res Complex Harwell, Rutherford Appleton Lab, Didcot OX11 0FA, Oxon, England. (creator_code:org_t)
- American Association for the Advancement of Science (AAAS), 2021
- 2021
- Engelska.
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Ingår i: Science Advances. - : American Association for the Advancement of Science (AAAS). - 2375-2548. ; 7:34
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://www.science....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Isopenicillin N synthase (IPNS) catalyzes the unique reaction of L-delta-(alpha-aminoadipoyl)-L-cysteinyl-D-valine (ACV) with dioxygen giving isopenicillin N (IPN), the precursor of all natural penicillins and cephalosporins. X-ray free-electron laser studies including time-resolved crystallography and emission spectroscopy reveal how reaction of IPNS:Fe(II):ACV with dioxygen to yield an Fe(III) superoxide causes differences in active site volume and unexpected conformational changes that propagate to structurally remote regions. Combined with solution studies, the results reveal the importance of protein dynamics in regulating intermediate conformations during conversion of ACV to IPN. The results have implications for catalysis by multiple IPNS-related oxygenases, including those involved in the human hypoxic response, and highlight the power of serial femtosecond crystallography to provide insight into long-range enzyme dynamics during reactions presently impossible for nonprotein catalysts.
Ämnesord
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
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- Av författaren/redakt...
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Rabe, Patrick
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Kamps, Jos J. A. ...
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Sutherlin, Kyle ...
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Linyard, James D ...
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Aller, Pierre
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Pham, Cindy C.
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visa fler...
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Makita, Hiroki
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Clifton, Ian
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McDonough, Micha ...
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Leissing, Thomas ...
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Shutin, Denis
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Lang, Pauline A.
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Butryn, Agata
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Brem, Jurgen
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Gul, Sheraz
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Fuller, Franklin ...
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Kim, In-Sik
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Cheah, Mun Hon
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Fransson, Thomas
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Bhowmick, Asmit
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Young, Iris D.
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O'Riordan, Lee
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Brewster, Aaron ...
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Pettinati, Ilari ...
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Doyle, Margaret
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Joti, Yasumasa
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Owada, Shigeki
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Tono, Kensuke
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Batyuk, Alexande ...
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Hunter, Mark S.
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Alonso-Mori, Rob ...
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Bergmann, Uwe
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Owen, Robin L.
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Sauter, Nicholas ...
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Claridge, Timoth ...
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Robinson, Carol ...
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Yachandra, Vitta ...
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Yano, Junko
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Kern, Jan F.
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Orville, Allen M ...
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Schofield, Chris ...
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