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In situ imaging rev...
In situ imaging reveals disparity between prostaglandin localization and abundance of prostaglandin synthases
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- Duncan, Kyle D. (författare)
- Uppsala universitet,Institutionen för kemi - BMC
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- Sun, Xiaofei (författare)
- Univ Cincinnati, Coll Med, Cincinnati, OH 45221 USA.
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- Baker, Erin S. (författare)
- North Carolina State Univ, Dept Chem, Raleigh, NC USA.
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- Dey, Sudhansu K. (författare)
- Univ Cincinnati, Coll Med, Cincinnati, OH 45221 USA.
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- Lanekoff, Ingela, Assoc. Prof. 1975- (författare)
- Uppsala universitet,Analytisk kemi
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(creator_code:org_t)
- 2021-08-13
- 2021
- Engelska.
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Ingår i: Communications Biology. - : Springer Nature. - 2399-3642. ; 4:1
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Duncan et al. use a mass spectrometry imaging method to assess the localization and concentration of prostaglandins (PGs) in mouse tissues during pregnancy. This study brings new biological insights into the spatial evaluation of PGs in tissues, which could reveal the functional significance of each PGs during different stages of embryo development/pregnancy. Prostaglandins are important lipids involved in mediating many physiological processes, such as allergic responses, inflammation, and pregnancy. However, technical limitations of in-situ prostaglandin detection in tissue have led researchers to infer prostaglandin tissue distributions from localization of regulatory synthases, such as COX1 and COX2. Herein, we apply a novel mass spectrometry imaging method for direct in situ tissue localization of prostaglandins, and combine it with techniques for protein expression and RNA localization. We report that prostaglandin D-2, its precursors, and downstream synthases co-localize with the highest expression of COX1, and not COX2. Further, we study tissue with a conditional deletion of transformation-related protein 53 where pregnancy success is low and confirm that PG levels are altered, although localization is conserved. Our studies reveal that the abundance of COX and prostaglandin D-2 synthases in cellular regions does not mirror the regional abundance of prostaglandins. Thus, we deduce that prostaglandins tissue localization and abundance may not be inferred by COX or prostaglandin synthases in uterine tissue, and must be resolved by an in situ prostaglandin imaging.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
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- art (ämneskategori)
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